@article{fdi:010077016,
  title = {{R}apid diagnostic tests as a source of dengue virus {RNA} for envelope gene amplification : a proof of concept},
  author = {{C}assidy-{S}eyoum, {S}. and {V}ongsouvath, {M}. and {S}engvilaipaseuth, {O}. and {S}eephonelee, {M}. and {B}harucha, {T}. and {L}amballerie, {X}. de and {N}ewton, {P}. {N}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey},
  editor = {},
  language = {{ENG}},
  abstract = {{M}olecular epidemiological data are key for dengue outbreak characterization and preparedness. {H}owever, sparse {D}engue virus ({DENV}) molecular information is available in {L}aos because of limited resources. {I}n this proof-of-concept study, we evaluated whether {DENV}1 {RNA} extracted from rapid diagnostic tests ({RDT}s) could be amplified and sequenced. {T}he protocol for envelope gene amplification from {RNA} purified from {RDT}s was first assessed using viral isolate dilutions then conducted using 14 dengue patient sera. {E}nvelope gene amplification was successful from patient sera with high virus titer, as was sequencing but with lower efficiency. {H}ence, based on our results, {RDT}s can be a source of {DENV}1 {RNA} for subsequent envelope gene amplification and sequencing. {T}his is a promising tool for collecting molecular epidemiology data from rural dengue-endemic areas. {H}owever, further investigations are needed to improve assay efficiency and to assess this tool's level of efficacy on a larger scale in the field.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {101},
  numero = {2},
  pages = {451--455},
  ISSN = {0002-9637},
  year = {2019},
  DOI = {10.4269/ajtmh.18-0831},
  URL = {https://www.documentation.ird.fr/hor/fdi:010077016},
}