Nebbak A. auteur aut IRD Dahmana H. auteur aut IRD Almeras L. auteur aut IRD Raoult D. auteur aut IRD Boulanger N. auteur aut IRD Jaulhac B. auteur aut IRD Mediannikov Oleg auteur aut IRD Parola P. auteur aut IRD text journalArticle eng text/pdf born digital access Fifty nymphal Ixodes ricinus ticks collected in Alsace, France, identified by morphological criteria and using MALDI-TOF MS, were tested by PCR to detect tick-associated bacteria and protozoan parasites. Seventy percent (35/50) of ticks contained at least one microorganism; 26% (9/35) contained two or more species. Several human pathogens were identified including Borrelia burgdorferi s.s. (4%), Borrelia afzelii (2%), Borrelia garinii (2%), Borrelia valaisiana (4%), Borrelia miyamotoi (2%), Rickettsia helvetica (6%) and "Babesia venatorum" (2%). Bartonella spp. (10%) and a Wolbachia spp. (8%) were also detected. The most common co-infections involved Anaplasmataceae with Borrelia spp. (4%), Anaplasmataceae with Bartonella spp. (6%) and Anaplasmataceae with Rickettsia spp. (6%). Co-infection involving three different groups of bacteria was seen between bacteria of the family Anaplasmataceae, Borrelia spp. and Bartonella spp. (2%). Results highlight the panel of infectious agents carried by Ixodes ricinus. Co-infection suggests the possibility of transmission of more than one pathogen to human and animals during tick blood feeding. specialized Ixodes ricinus Co-infection Bacterial pathogens Protozoan parasites MALDI-TOF MS FRANCE ALSACE 052 10 6 art. 101241 [5 p.] 2019 1877-959X https://www.documentation.ird.fr/hor/fdi:010077009 10.1016/j.ttbdis.2019.06.001 1877-959X [F B010077009] https://www.documentation.ird.fr/hor/fdi:010077009 https://www.documentation.ird.fr/intranet/publi/2019/10/010077009.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-11-05 2025-02-24 fdi:010077009 fre