Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Ticks and Tick-Borne Diseases art. 101241 [5 p.] Ixodes ricinus Co-infection Bacterial pathogens Protozoan parasites MALDI-TOF MS FRANCE ALSACE 2019 fdi:010077009 ENG Ticks and Tick-Borne Diseases 1877-959X ISI:000484783700001 6 10.1016/j.ttbdis.2019.06.001 https://www.documentation.ird.fr/hor/fdi:010077009 https://www.documentation.ird.fr/intranet/publi/2019/10/010077009.pdf 10 Horizon (IRD) Fifty nymphal Ixodes ricinus ticks collected in Alsace, France, identified by morphological criteria and using MALDI-TOF MS, were tested by PCR to detect tick-associated bacteria and protozoan parasites. Seventy percent (35/50) of ticks contained at least one microorganism; 26% (9/35) contained two or more species. Several human pathogens were identified including Borrelia burgdorferi s.s. (4%), Borrelia afzelii (2%), Borrelia garinii (2%), Borrelia valaisiana (4%), Borrelia miyamotoi (2%), Rickettsia helvetica (6%) and "Babesia venatorum" (2%). Bartonella spp. (10%) and a Wolbachia spp. (8%) were also detected. The most common co-infections involved Anaplasmataceae with Borrelia spp. (4%), Anaplasmataceae with Bartonella spp. (6%) and Anaplasmataceae with Rickettsia spp. (6%). Co-infection involving three different groups of bacteria was seen between bacteria of the family Anaplasmataceae, Borrelia spp. and Bartonella spp. (2%). Results highlight the panel of infectious agents carried by Ixodes ricinus. Co-infection suggests the possibility of transmission of more than one pathogen to human and animals during tick blood feeding. 052 UR257 Algérie