@article{fdi:010076688,
  title = {{R}hizobium sophorae, {R}hizobium laguerreae, and two novel {R}hizobium genospecies associated with {V}icia sativa {L}. in {N}orthwest {C}hina},
  author = {{Z}hang, {J}. {J}. and {S}hang, {Y}. {M}. and {P}eng, {S}. {S}. and {C}hen, {W}. {F}. and {W}ang, {E}. {T}. and {L}ajudie, {P}hilippe de and {L}i, {B}. {Y}. and {G}uo, {C}. and {L}iu, {C}. {Z}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ims {T}o identify the rhizobia nodulating {V}icia sativa in {N}orthwestern {C}hina and to estimate their geographic distribution. {M}ethods {R}hizobia trapped with {V}. sativa plants from soils at six sites in the northwest of {C}hina were classified into genotypes by {PCR}-based restriction fragment length polymorphism ({RFLP}) of 16{S}-23{S} r{RNA} intergenic spacer ({IGS}) and 16{S} r{RNA} genes, and phylogenetic analyses of housekeeping (16{S} r{RNA}, rec{A}, atp{D}) and symbiotic genes were performed for the representative strains. {S}oil physicochemical characteristics were recorded and canonical correlation analysis was performed to examine the correlations between soil features and distribution of rhizobial genotypes. {R}esults {A} total of 202 rhizobial isolates were discriminated by {RFLP} analyses into 15 {IGS} types and a single 16{S} r{RNA} type, which were identified as {R}hizobium by 16{S} r{RNA} gene phylogeny and as four clusters by multilocus sequence analysis ({MLSA}). {C}luster 1 covering 86 strains and 7 {IGS} types prevalent in all sites was identified as {R}hizobium laguerreae; cluster 2 was {R}. sophorae with 18 strains in 2 {IGS} types found in the site {Q}-{GD}. {E}ach of cluster 3 and cluster 4 contained three {IGS} types representing two novel {R}hizobium genospecies specific to {G}ansu {P}rovince and {S}hanxi {P}rovince, respectively. {F}our nod{C} phylogenetic clades were defined among the isolates. {C}onclusions {R}. sophorae, {R}. laguerreae, and two novel {R}hizobium genospecies with diverse symbiotic genotypes are associated with {V}icia sativa {L}. in {N}orthwest {C}hina. {T}heir biogeographic patterns are mainly directed by soil p{H} and salinity. {T}his is the first study on the diversity of rhizobia nodulating {V}icia sativa in {C}hina.},
  keywords = {{V}icia sativa ; {R}hizobium sophorae ; {R}hizobium laguerreae ; {R}hizobium spp. nov. ; {N}orthwest {C}hina ; {CHINE}},
  booktitle = {},
  journal = {{P}lant and {S}oil},
  volume = {442},
  numero = {1-2},
  pages = {113--126},
  ISSN = {0032-079{X}},
  year = {2019},
  DOI = {10.1007/s11104-019-04168-w},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076688},
}