@article{fdi:010076646,
  title = {{T}ranscriptomic dissection of {B}radyrhizobium sp. strain {ORS}285 in symbiosis with {A}eschynomene spp. inducing different bacteroid morphotypes with contrasted symbiotic efficiency},
  author = {{L}amouche, {F}. and {G}ully, {D}jamel and {C}haumeret, {A}. and {N}ouwen, {N}ico and {V}erly, {C}. and {P}ierre, {O}. and {S}ciallano, {C}. and {F}ardoux, {J}o{\¨e}l and {J}eudy, {C}. and {S}zucs, {A}. and {M}ondy, {S}. and {S}alon, {C}. and {N}agy, {I}. and {K}ereszt, {A}. and {D}essaux, {Y}. and {G}iraud, {E}ric and {M}ergaert, {P}. and {A}lunni, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o circumvent the paucity of nitrogen sources in the soil legume plants establish a symbiotic interaction with nitrogen-fixing soil bacteria called rhizobia. {D}uring symbiosis, the plants form root organs called nodules, where bacteria are housed intracellularly and become active nitrogen fixers known as bacteroids. {D}epending on their host plant, bacteroids can adopt different morphotypes, being either unmodified ({U}), elongated ({E}) or spherical ({S}). {E}- and {S}-type bacteroids undergo a terminal differentiation leading to irreversible morphological changes and {DNA} endoreduplication. {P}revious studies suggest that differentiated bacteroids display an increased symbiotic efficiency ({E} > {U} and {S} > {U}). {I}n this study, we used a combination of {A}eschynomene species inducing {E}- or {S}-type bacteroids in symbiosis with {B}radyrhizobium sp. {ORS}285 to show that {S}-type bacteroids present a better symbiotic efficiency than {E}-type bacteroids. {W}e performed a transcriptomic analysis on {E}- and {S}-type bacteroids formed by {A}eschynomene afraspera and {A}eschynomene indica nodules and identified the bacterial functions activated in bacteroids and specific to each bacteroid type. {E}xtending the expression analysis in {E}- and {S}-type bacteroids in other {A}eschynomene species by q{RT}-{PCR} on selected genes from the transcriptome analysis narrowed down the set of bacteroid morphotype-specific genes. {F}unctional analysis of a selected subset of 31 bacteroid-induced or morphotype-specific genes revealed no symbiotic phenotypes in the mutants. {T}his highlights the robustness of the symbiotic program but could also indicate that the bacterial response to the plant environment is partially anticipatory or even maladaptive. {O}ur analysis confirms the correlation between differentiation and efficiency of the bacteroids and provides a framework for the identification of bacterial functions that affect the efficiency of bacteroids.(c) 2018 {S}ociety for {A}pplied {M}icrobiology and {J}ohn {W}iley & {S}ons {L}td},
  keywords = {},
  booktitle = {},
  journal = {{E}nvironmental {M}icrobiology},
  volume = {21},
  numero = {9},
  pages = {3244--3258},
  ISSN = {1462-2912},
  year = {2019},
  DOI = {10.1111/1462-2920.14292},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076646},
}