Kolia-Diafouka P. auteur aut IRD Carrere-Kremer S. auteur aut IRD Lounnas Manon auteur aut IRD Bourdin A. auteur aut IRD Kremer L. auteur aut IRD Van de Perre P. auteur aut IRD Godreuil S. auteur aut IRD Tuaillon E. auteur aut IRD text journalArticle eng text/pdf born digital access The Xpert MTB/RIF Ultra assay has recently been launched to improve the detection of smear negative disease. This retrospective study compares the sensitivity of Xpert MTB/RIF Ultra with that of Xpert MTB/RIF tests and IS6110 real-time PCR in sputum. Diagnostic performance of three molecular tests was evaluated using 48 culture-positive clinical respiratory specimens diluted to obtain paucibacillary sputum specimens. Xpert MTB/RIF Ultra had the highest sensitivity of 100% compared to 42% (P < 0.001) for Xpert MTB/RIF and 64.5% (P = 0.02) for IS6110-PCR. All "very low" or "low" positive specimens using Xpert MTB/RIF Ultra were tested positive using IS6110-PCR, but 35.4% were found negative using Xpert MTB/RIF. Xpert MTB/RIF Ultra is more sensitive than the two other tests for sputum with a low bacterial load. Adding detection of IS6110 and IS1081 to rpoB, is a key evolution of the assay and improves the detection of Mycobacterium tuberculosis' DNA in paucibacillary sputum. specialized Chelex (R) Mycobacterium tuberculosis IS6110-PCR Xpert MTB/RIF Xpert MTB/RIF ultra Paucibacillary sputum 084 050 94 4 365-370 2019 0732-8893 https://www.documentation.ird.fr/hor/fdi:010076533 10.1016/j.diagmicrobio.2019.02.008 0732-8893 [F B010076533] https://www.documentation.ird.fr/hor/fdi:010076533 https://www.documentation.ird.fr/intranet/publi/2019/08/010076533.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-09-19 2025-02-24 fdi:010076533 fre