%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES %A Kolia-Diafouka, P. %A Carrere-Kremer, S. %A Lounnas, Manon %A Bourdin, A. %A Kremer, L. %A Van de Perre, P. %A Godreuil, S. %A Tuaillon, E. %T Detection of Mycobacterium tuberculosis in paucibacillary sputum : performances of the Xpert MTB/RIF ultra compared to the Xpert MTB/RIF, and IS6110 PCR %D 2019 %L fdi:010076533 %G ENG %J Diagnostic Microbiology and Infectious Disease %@ 0732-8893 %K Chelex (R) ; Mycobacterium tuberculosis ; IS6110-PCR ; Xpert MTB/RIF ; Xpert ; MTB/RIF ultra ; Paucibacillary sputum %M ISI:000478701800011 %N 4 %P 365-370 %R 10.1016/j.diagmicrobio.2019.02.008 %U https://www.documentation.ird.fr/hor/fdi:010076533 %> https://www.documentation.ird.fr/intranet/publi/2019/08/010076533.pdf %V 94 %W Horizon (IRD) %X The Xpert MTB/RIF Ultra assay has recently been launched to improve the detection of smear negative disease. This retrospective study compares the sensitivity of Xpert MTB/RIF Ultra with that of Xpert MTB/RIF tests and IS6110 real-time PCR in sputum. Diagnostic performance of three molecular tests was evaluated using 48 culture-positive clinical respiratory specimens diluted to obtain paucibacillary sputum specimens. Xpert MTB/RIF Ultra had the highest sensitivity of 100% compared to 42% (P < 0.001) for Xpert MTB/RIF and 64.5% (P = 0.02) for IS6110-PCR. All "very low" or "low" positive specimens using Xpert MTB/RIF Ultra were tested positive using IS6110-PCR, but 35.4% were found negative using Xpert MTB/RIF. Xpert MTB/RIF Ultra is more sensitive than the two other tests for sputum with a low bacterial load. Adding detection of IS6110 and IS1081 to rpoB, is a key evolution of the assay and improves the detection of Mycobacterium tuberculosis' DNA in paucibacillary sputum. %$ 084 ; 050