@article{fdi:010076519,
  title = {{E}nhancement of vitamin {B}-6 levels in rice expressing {A}rabidopsis vitamin {B}-6 biosynthesis de novo genes},
  author = {{M}angel, {N}. and {F}udge, {J}. {B}. and {L}i, {K}. {T}. and {W}u, {T}. {Y}. and {T}ohge, {T}. and {F}ernie, {A}. {R}. and {S}zurek, {B}oris and {F}itzpatrick, {T}. {B}. and {G}ruissem, {W}. and {V}anderschuren, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{V}itamin {B}-6 (pyridoxine) is vital for key metabolic reactions and reported to have antioxidant properties in planta. {T}herefore, enhancement of vitamin {B}-6 content has been hypothesized to be a route to improve resistance to biotic and abiotic stresses. {M}ost of the current studies on vitamin {B}-6 in plants are on eudicot species, with monocots remaining largely unexplored. {I}n this study, we investigated vitamin {B}-6 biosynthesis in rice, with a view to examining the feasibility and impact of enhancing vitamin {B}-6 levels. {C}onstitutive expression in rice of two {A}rabidopsis thaliana genes from the vitamin {B}-6 biosynthesis de novo pathway, {A}t{PDX}1.1 and {A}t{PDX}2, resulted in a considerable increase in vitamin {B}-6 in leaves (up to 28.3-fold) and roots (up to 12-fold), with minimal impact on general growth. {R}ice lines accumulating high levels of vitamin {B}-6 did not display enhanced tolerance to abiotic stress (salt) or biotic stress (resistance to {X}anthomonas oryzae infection). {W}hile a significant increase in vitamin {B}-6 content could also be achieved in rice seeds (up to 3.1-fold), the increase was largely due to its accumulation in seed coat and embryo tissues, with little enhancement observed in the endosperm. {H}owever, seed yield was affected in some vitamin {B}-6-enhanced lines. {N}otably, expression of the transgenes did not affect the expression of the endogenous rice {PDX} genes. {I}ntriguingly, despite transgene expression in leaves and seeds, the corresponding proteins were only detectable in leaves and could not be observed in seeds, possibly pointing to a mode of regulation in this organ.},
  keywords = {{PDX} proteins ; monocot ; rice ; crop ; vitamin {B}-6 ; stress},
  booktitle = {},
  journal = {{P}lant {J}ournal},
  volume = {99},
  numero = {6},
  pages = {1047--1065},
  ISSN = {0960-7412},
  year = {2019},
  DOI = {10.1111/tpj.14379},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076519},
}