@article{fdi:010076445,
  title = {{T}ranscriptomic profiling of {B}urkholderia phymatum {STM}815, {C}upriavidus taiwanensis {LMG}19424 and {R}hizobium mesoamericanum {STM}3625 in response to {M}imosa pudica root exudates illuminates the molecular basis of their nodulation competitiveness and symbiotic evolutionary history},
  author = {{K}lonowska, {A}gnieszka and {M}elkonian, {R}. and {M}ich{\'e}, {L}. and {T}isseyre, {P}ierre and {M}oulin, {L}ionel},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {R}hizobial symbionts belong to the classes {A}lphaproteobacteria and {B}etaproteobacteria (called "alpha" and "beta"-rhizobia). {M}ost knowledge on the genetic basis of symbiosis is based on model strains belonging to alpha-rhizobia. {M}imosa pudica is a legume that offers an excellent opportunity to study the adaptation toward symbiotic nitrogen fixation in beta-rhizobia compared to alpha-rhizobia. {I}n a previous study ({M}elkonian et al., {E}nviron {M}icrobiol 16:2099-111, 2014) we described the symbiotic competitiveness of {M}. pudica symbionts belonging to {B}urkholderia, {C}upriavidus and {R}hizobium species. {R}esults: {I}n this article we present a comparative analysis of the transcriptomes (by {RNA}seq) of {B}. phymatum {STM}815 ({BP}), {C}. taiwanensis {LMG}19424 ({CT}) and {R}. mesoamericanum {STM}3625 ({RM}) in conditions mimicking the early steps of symbiosis (i.e. perception of root exudates). {BP} exhibited the strongest transcriptome shift both quantitatively and qualitatively, which mirrors its high competitiveness in the early steps of symbiosis and its ancient evolutionary history as a symbiont, while {CT} had a minimal response which correlates with its status as a younger symbiont (probably via acquisition of symbiotic genes from a {B}urkholderia ancestor) and {RM} had a typical response of {A}lphaproteobacterial rhizospheric bacteria. {I}nterestingly, the upregulation of nodulation genes was the only common response among the three strains; the exception was an up-regulated gene encoding a putative fatty acid hydroxylase, which appears to be a novel symbiotic gene specific to {M}imosa symbionts. {C}onclusion: {T}he transcriptional response to root exudates was correlated to each strain nodulation competitiveness, with {B}urkholderia phymatum appearing as the best specialised symbiont of {M}imosa pudica.},
  keywords = {},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {19},
  numero = {},
  pages = {art. no 105 [22  ]},
  ISSN = {1471-2164},
  year = {2018},
  DOI = {10.1186/s12864-018-4487-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076445},
}