%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Chauvet, M.
%A Tétard, M.
%A Cottrell, Gilles
%A Aussenac, Florentin
%A Brossier, E.
%A Denoyel, L.
%A Lohezic, M.
%A Milet, Jacqueline
%A Tuikue Ndam, Nicaise
%A Pineau, D.
%A Roman, Jocelyne
%A Luty, Adrian
%A Gamain, B.
%A Migot Nabias, Florence
%A Merckx, A.
%T Impact of hemoglobin S trait on cell surface antibody recognition of Palsmodium falciparum - infected erythrocytes in pregnancy - associated malaria
%D 2019
%L fdi:010076291
%G ENG
%J Open Forum Infectious Diseases
%@ 2328-8957
%K PALUDISME ; IMMUNOLOGIE ; FEMME ; GROSSESSE ; BIOLOGIE MOLECULAIRE
%K CYTOMETRIE DE FLUX ; HEMOGLOBINE
%K BENIN
%M ISI:000474844200046
%N 4
%P ofz156 [8 ]
%R 10.1093/ofid/ofz156
%U https://www.documentation.ird.fr/hor/fdi:010076291
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-07/010076291.pdf
%V 6
%W Horizon (IRD)
%X Background: Sickle cell trait (HbAS) confers partial protection against malaria by reducing the adhesion of Plasmodium falciparum-infected erythrocytes to host receptors, but little is known about its potential protection against placental malaria. Methods: Using flow cytometry, we assessed the recognition of HbAA and HbAS VAR2CSA-expressing infected erythrocytes, by plasma from 159 Beninese pregnant women with either HbAA (normal) or HbAS. Using multivariate linear models adjusted for gravidity, parasite infection at delivery, glucose-6-phosphate dehydrogenase deficiency, and α-thalassemia carriage, we observed significantly reduced cell surface antibody binding of HbAS-infected erythrocytes by plasma from HbAS compared with HbAA women (P < 10–3). Results: The difference in cell surface antibody binding was only observed when infected erythrocytes and plasma were associated according to the same hemoglobin genotype. Similar levels of VAR2CSA-specific antibody were measured by enzyme-linked immunosorbent assay in the 2 groups, suggesting that the altered interaction between VAR2CSA and HbAS women's antibodies could reflect abnormal display of VAR2CSA on HbAS erythrocytes. Conclusions: Our data stress the need for assessments of erythrocyte disorders such as the sickle cell trait in a population group when studying immunological responses to P falciparum.
%$ 052ANOPAL04 ; 020BIOL ; 050MEDECI