@article{fdi:010076227,
  title = {{G}enetic diversity of rhizobia associated with root nodules of white lupin ({L}upinus albus {L}.) in {T}unisian calcareous soils},
  author = {{T}ounsi-{H}ammami, {S}. and {L}e {R}oux, {C}. and {D}hane-{F}itouri, {S}. and {L}ajudie, {P}hilippe de and {D}uponnois, {R}obin and {B}en {J}eddi, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}ith a view to introducing white lupin ({L}upinus albus {L}.) for cultivation in {T}unisian calcareous soils, compatible indigenous rhizobia for nitrogen-fixing symbiosis were investigated and characterized. {T}wo {L}. albus varieties, {M}ekna and {L}umen, were used to trap rhizobia in soil samples collected from 56 sites with high active lime contents (0-49%). {N}odulation occurred in only 15 soils. {T}he local variety, {M}ekna, developed significantly more root nodules and had a trapping capacity in more soils than the imported variety {L}umen. {A} phylogenetic analysis based on the partial 16{S}-23{S} ribosomal {RNA} internal transcribed spacer region ({ITS}) and multi-locus sequence analysis ({MLSA}) of three chromosomal housekeeping genes, rec{A}, atp{D} and dna{K}, showed that strains were affiliated to {A}grobacterium, {R}hizobium, and {N}eorhizobium, with large internal diversity, including separate lineages. {I}nfectivity tests highlighted some nodulation specificity at the plant variety level, since the strains originating from {M}ekna could only nodulate this variety, while strains trapped in {L}umen could nodulate both varieties. {W}hen inoculated, almost all strains resulted in a significant increase in plant shoot dry weight on {L}. albus. {A}lthough {A}grobacterium sp. strains isolated from {L}. albus could nodulate and had a plant growth promoting effect, no nod{A} and nod{C} genes could be amplified. {T}his is discussed together with the absence of bradyrhizobia and the general infrequency of {L}. albus-nodulating rhizobia in {T}unisian soils. {T}he adapted and efficient rhizobial strains reported here were promising candidates for inoculant development and represent a contribution towards successful cultivation of {L}. albus in {T}unisia, especially the most promising {M}ekna variety.},
  keywords = {{L}upinus albus ; {C}alcareous soils ; {A}grobacterium ; {MLSA} ; {R}hizobium ; {N}eorhizobium ; {TUNISIE} ; {ZONE} {MEDITERRNANEENNE}},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {42},
  numero = {4},
  pages = {448--456},
  ISSN = {0723-2020},
  year = {2019},
  DOI = {10.1016/j.syapm.2019.04.002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076227},
}