@article{fdi:010076207,
  title = {{I}nterferon-inducible protein (ifi) 16 regulates chikungunya and zika virus infection in human skin fibroblasts},
  author = {{W}ichit, {S}. and {H}amel, {R}odolphe and {Y}ainoy, {S}. and {G}umpangseth, {N}. and {P}anich, {S}. and {P}huadraksa, {T}. and {S}aetear, {P}. and {M}onteil, {A}. and {V}argas, {R}. {M}. and {M}iss{\'e}, {D}oroth{\'e}e},
  editor = {},
  language = {{ENG}},
  abstract = {{C}hikungunya virus ({CHIKV}), a re-emerging infectious arbovirus, causes {C}hikungunya fever that is characterized by fever, skin rash, joint pain, arthralgia and occasionally death. {D}espite it has been described for 66 years already, neither potential vaccine nor a specific drug is available yet. {D}uring {CHIKV} infection, interferon type {I} signaling pathway is stimulated and releases hundreds of interferon stimulated genes ({ISG}s). {O}ur previous study reported that {IFI}16, a member of {ISG}s, is up-regulated during {CHIKV} virus infection and the suppression of the gene resulted in increased virus replication. {F}urthermore, our group also found that inflammasome activation can inhibit {CHIKV} infection in human foreskin cells ({HFF}1). {C}oncomitantly, it has been reported that {IFI}16 activates the inflammasome to suppress virus infection. {T}herefore, we have hypothesized that {IFI}16 could be involved in {CHIKV} infection. {I}n this study, we confirmed the expression level of {IFI}16 by {W}estern blotting analysis and found that {IFI}16 was up-regulated following {CHIKV} infection in both {HFF}1 and human embryonic kidney cells. {W}e next investigated its antiviral activity and found that forced expression of {IFI}16 completely restricted {CHIKV} infection while endogenous silencing of the gene markedly increased virus replication. {F}urthermore, we have discovered that {IFI}16 inhibited {CHIKV} replication, at least, in cell-to-cell transmission as well as the diffusion step. {I}nterestingly, {IFI}16 also exerted its antiviral activity against {Z}ika virus ({ZIKV}) infection, the global threat reemerging virus can cause microcephaly in humans. {T}aken together, this study provides the first evidence of an antivirus activity of {IFI}16 during in vitro arbovirus infection, thus expanding its antiviral spectrum that paves the way to further development of antiviral drugs and vaccines.},
  keywords = {{C}hikungunya virus ; {Z}ika virus ; {IFI}16 ; human skin fibroblasts ; antiviral},
  booktitle = {},
  journal = {{E}xcli {J}ournal},
  volume = {18},
  numero = {},
  pages = {467--476},
  ISSN = {1611-2156},
  year = {2019},
  DOI = {10.17179/excli2019-1271},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076207},
}