Ferraris Pauline auteur aut IRD Cochet M. auteur aut IRD Hamel Rodolphe auteur aut IRD Gladwyn-Ng I. auteur aut IRD Alfan C. auteur aut IRD Diop F. auteur aut IRD Garcia Déborah auteur aut IRD Talignani L. auteur aut IRD Montero-Menei C. N. auteur aut IRD Nougairede A. auteur aut IRD Yssel H. auteur aut IRD Nguyen L. auteur aut IRD Coulpier M. auteur aut IRD Missé Dorothée auteur aut IRD text journalArticle eng text/pdf reformatted digital access Zika virus (ZIKV) is a mosquito-borne Flavivirus that causes Zika disease with particular neurological complications, including Guillain-Barre Syndrome and congenital microcephaly. Although ZIKV has been shown to directly infect human neural progenitor cells (hNPCs), thereby decreasing their viability and growth, it is as yet unknown which of the cellular pathways involved in the disruption of neurogenesis are affected following ZIKV infection. By comparing the effect of two ZIKV strains in vitro on hNPCs, the differentiation process of the latter cells was found to lead to a decreased susceptibility to infection and cell death induced by each of the ZIKV strains, which was associated with an earlier and stronger antiviral innate immune response in infected, differentiated hNPCs, as compared to undifferentiated cells. Moreover, ZIKV modulated, both in hNPCs and in vivo in fetal brain in an experimental mouse model, the expression of the Notch pathway which is involved in cellular proliferation, apoptosis and differentiation during neurogenesis. These results show that the differentiation state of hNPCs is a significant factor contributing to the outcome of ZIKV infection and furthermore suggest that ZIKV infection might initiate early activation of the Notch pathway resulting in an abnormal differentiation process, implicated in ZIKV-induced brain injury. specialized Zika arbovirus human neural progenitor Notch neurogenesis flavivirus 052 050 8 1 1003-1016 2019 2222-1751 https://www.documentation.ird.fr/hor/fdi:010076199 10.1080/22221751.2019.1637283 2222-1751 [F B010076199] https://www.documentation.ird.fr/hor/fdi:010076199 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-07/010076199.pdf IRD - Base Horizon / Pleins textes 2019-08-05 2025-02-24 fdi:010076199 fre