@article{fdi:010076148,
  title = {{A}n optimized microsatellite scheme for assessing populations of {X}anthomonas phaseoli pv. manihotis},
  author = {{R}ache, {L}. and {B}londin, {L}. and {F}lores, {C}. and {T}rujillo, {C}. and {S}zurek, {B}oris and {R}estrepo, {S}. and {K}oebnik, {R}alf and {B}ernal, {A}. and {V}erniere, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{D}iverse molecular markers have been used to analyze the genetic diversity of plant pathogens. {C}ompared with traditional fingerprinting methods, multiple loci variable number of tandem repeat analyses ({MLVA}s) have gained importance recently due to their reproducibility, high discriminatory power, ease of performance, low cost, and throughput potential. {T}hese characteristics are desirable for continuous pathogen monitoring, especially for pathogens with relatively low genetic diversity, and for disease epidemiology studies. {G}enetic diversity studies of {X}anthomonas phaseoli pv. manihotis, which is the causal agent of cassava bacterial blight, have shown variability and changes in the bacterial population over time. {T}hus, an easy and fast method needs to be developed to type populations of this pathogen in different countries of the world, especially on small scales. {I}n this study, we developed an {MLVA} scheme to analyze {X}. phaseoli pv. manihotis variability on a local scale. {T}he {MLVA}-15 scheme comprises 15 variable number of tandem repeat loci grouped into four multiplex polymerase chain reaction pools. {W}e showed that the {MLVA}-15 scheme had slightly higher discriminatory ability at the locality level when compared with amplified fragment length polymorphisms. {T}he {MLVA}-15 scheme allowed for an accurate determination of the number of genotypes in the sample and showed reproducibility and portability. {A}dditionally, this scheme could be used to analyze numerous strains in a reasonable timeframe. {T}he {MLVA}-15 scheme was highly specific to {X}. phaseoli but up to eight tandem repeat loci could be amplified from other {X}anthomonas spp. {F}inally, we assessed the utility of the scheme for analyses of {X}. phaseoli pv. manihotis genetic variability in the {C}olombian {C}aribbean region. {MLVA}-15 distinguished 88.9% of the haplotypes in our sample. {S}trains originating from the same field and isolated at the same time could be discriminated. {I}n this study, the advantages of the {MLVA}-15 scheme targeting 6- or 7-bp repeats were demonstrated. {M}oreover, this scheme was a fast method that was appropriate for routine monitoring of {X}. phaseoli pv. manihotis populations on a local scale and, thus, was useful for addressing epidemiological questions.},
  keywords = {epidemiology ; molecular marker ; pathogen diversity ; tandem repeat ; {X}anthomonas ; {COLOMBIE} ; {CARRAIBE}},
  booktitle = {},
  journal = {{P}hytopathology},
  volume = {109},
  numero = {5},
  pages = {859--869},
  ISSN = {0031-949{X}},
  year = {2019},
  DOI = {10.1094/phyto-06-18-0210-r},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076148},
}