@article{fdi:010076112,
  title = {{S}mall {RNA}-seq reveals novel regulatory components for apomixis in {P}aspalum notatum},
  author = {{O}rtiz, {J}. {P}. {A}. and {L}eblanc, {O}livier and {R}ohr, {C}. and {G}risolia, {M}. and {S}iena, {L}. {A}. and {P}odio, {M}. and {C}olono, {C}. and {A}zzaro, {C}. and {P}essino, {S}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{A}pomixis is considered an evolutionary deviation of the sexual reproductive pathway leading to the generation of clonal maternal progenies by seeds. {R}ecent evidence from model and non-model species suggested that this trait could be modulated by epigenetic mechanisms involving small {RNA}s (s{RNA}s). {H}ere we profiled floral s{RNA}s originated from apomictic and sexual {P}aspalum notatum genotypes in order to identify molecular pathways under epigenetic control that might be involved in the transition from sexuality to agamospermy.{R}esults{T}he mining of genes participating in s{RNA}-directed pathways from floral {P}aspalum transcriptomic resources showed these routes are functional during reproductive development, with several members differentially expressed in apomictic and sexual plants. {T}riplicate floral s{RNA} libraries derived from apomictic and a sexual genotypes were characterized by using high-throughput sequencing technology. {E}dge{R} was apply to compare the number of s{RNA} reads between sexual and apomictic libraries that map over all {P}aspalum floral transcripts. {A} total of 1525 transcripts showed differential s{RNA} representation, including genes related to meiosis, plant hormone signaling, biomolecules transport, transcription control and cell cycle. {S}urvey for mi{RNA} precursors on transcriptome and genome references allowed the discovery of 124 entities, including 40 conserved and 8 novel ones. {F}ifty-six clusters were differentially represented in apomictic and sexual plants. {A}ll differentially expressed mi{RNA}s were up-regulated in apomictic libraries but mi{R}2275, which showed different family members with opposed representation. {E}xamination of predicted mi{RNA}s targets detected 374 potential candidates. {C}onsidering s{RNA}, mi{RNA}s and target surveys together, 14 genes previously described as related with auxin metabolism, transport and signaling were detected, including {AMINO} {ACID}/{AUXIN} {PERMEASE} 15, {IAA}-{AMIDO} {SYNTHETASE} {GH}3-8, {IAA}30, mi{R}160, mi{R}167, mi{R}164, mi{R}319, {ARF}2, {ARF}8, {ARF}10, {ARF}12, {AFB}2, {PROLIFERATING} {CELL} {FACTOR} 6 and {NITRATE} {TRANSPORTER} 1.1.{C}onclusions{T}his work provides a comprehensive survey of the s{RNA} differential representation in flowers of sexual and apomictic {P}aspalum notatum plants. {A}n integration of the small {RNA} profiling data presented here and previous transcriptomic information suggests that s{RNA}-mediated regulation of auxin pathways is pivotal in promoting apomixis. {T}hese results will underlie future functional characterization of the molecular components mediating the switch from sexuality to apomixis.},
  keywords = {{A}pomixis ; {A}pospory ; {A}uxin ; mi{RNA} ; {P}lant reproduction ; s{RNA}},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {20},
  numero = {},
  pages = {art. 487 [17 p.]},
  ISSN = {1471-2164},
  year = {2019},
  DOI = {10.1186/s12864-019-5881-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076112},
}