@article{fdi:010076104,
  title = {{O}streopsis lenticularis {Y}. {F}ukuyo ({D}inophyceae, {G}onyaulacales) from {F}rench {P}olynesia ({S}outh {P}acific {O}cean) : a revisit of its morphology, molecular phylogeny and toxicity},
  author = {{C}hom{\'e}rat, {N}. and {B}ilien, {G}. and {D}errien, {A}. and {H}enry, {K}. and {U}ng, {A}. and {V}iallon, {J}. and {D}arius, {H}. {T}. and {G}atti, {C}. {M}. {I}. and {R}ou{\'e}, {M}{\'e}lanie and {H}erv{\'e}, {F}. and {R}eveillon, {D}. and {A}mzil, {Z}. and {C}hinain, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o date, the genus {O}streopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. {T}he taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. {T}he species {O}streopsis lenticularis was first described by {Y}. {F}ukuyo from {F}rench {P}olynesia using light microscopy observations, but without genetic information associated. {T}he present study aims at revisiting the morphology, molecular phylogeny and toxicity of {O}. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of {F}rench {P}olynesia, namely the {S}ociety, {A}ustrales, {M}arquesas and {G}ambier archipelagos. {O}bservations in light, epifluorescence and field emission scanning electron microscopy of several of these strains analyzed revealed morphological features in perfect agreement with the original description of {O}. lenticularis. {C}ells were oval, not undulated, 60.5-94.4 mu m in dorso-ventral length, 56.1-78.2 mu m in width, and possessed a typical plate pattern with thecal plates showing two sizes of pores. {P}hylogenetic analyses inferred from the {LSU} r{DNA} and {ITS}-5.8{S} sequences revealed that the 47 strains correspond to a single genotype, clustering with a strong support with sequences previously ascribed to {O}streopsis sp. 5. {C}lonal cultures of {O}. lenticularis were also established and further tested for their toxicity using the neuroblastoma cell-based assay and {LCMS}/{MS} analyses. {N}one of the 19 strains tested showed toxic activity on neuroblastoma cells, while {LCMS}/{MS} analyses performed on the strains from {T}ahiti {I}sland (i.e. type locality) confirmed that palytoxin and related structural analogs were below the detection limit. {T}hese findings allow to clarify unambiguously the genetic identity of {O}. lenticularis while confirming previous results from the {W}estern {P}acific which indicate that this species shows no toxicity, thus stressing the need to reconsider its current classification within the group of toxic species.},
  keywords = {{ITS}-5.8{S} r{DNA} ; {LSU} r{DNA} ; {M}icroscopy ; {O}streopsis lenticularis ; {T}axonomy ; {T}oxins ; {POLYNESIE} {FRANCAISE} ; {TAHITI}},
  booktitle = {},
  journal = {{H}armful {A}lgae},
  volume = {84},
  numero = {},
  pages = {95--111},
  ISSN = {1568-9883},
  year = {2019},
  DOI = {10.1016/j.hal.2019.02.004},
  URL = {https://www.documentation.ird.fr/hor/fdi:010076104},
}