@article{fdi:010075955,
  title = {{A}queous extracts from {U}ncaria tomentosa ({W}illd. ex {S}chult.) {DC}. reduce bronchial hyperresponsiveness and inflammation in a murine model of asthma},
  author = {{C}estari {A}zevedo, {B}. and {J}unqueira {F}reitas {M}orel, {L}. and {C}armona, {F}. and {M}attar {C}unha, {T}. and {T}aleb {C}ontini, {S}.{H}. and {D}elprete, {P}iero and et al.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}thnopharmacological relevance: {U}ncaria tomentosa ({W}illd. {E}x {S}chult) {DC} is used by indigenous tribes in the {A}mazonian region of {C}entral and {S}outh {A}merica to treat inflammation, allergies and asthma. {T}he therapeutic properties of {U}. tomentosa have been attributed to the presence of tetracyclic and pentacyclic oxindole alkaloids and to phenolic acids.{A}ims of the study: {T}o characterize aqueous bark extracts ({ABE}) and aqueous leaf extracts ({ALE}) of {U}. tomentosa and to compare their anti-inflammatory effects. {M}aterials and methods: {C}onstituents of the extracts were identified by ultra performance liquid chromatography-mass spectrometry. {A}nti-inflammatory activities were assessed in vitro by exposing lipopolysaccharide-stimulated macrophage cells ({RAW}264.7-{L}uc) to {ABE}, {ALE} and standard mitraphylline. {I}n vivo assays were performed using a murine model of ovalbumin ({OVA})-induced asthma. {OVA}-sensitized animals were treated with {ABE} or {ALE} while controls received dexamethasone or saline solution. {B}ronchial hyperresponsiveness, production of {T}h1 and {T}h2 cytokines, total and differential counts of inflammatory cells in the bronchoalveolar lavage ({BAL}) and lung tissue were determined. {R}esults: {M}itraphylline, isomitraphylline, chlorogenic acid and quinic acid were detected in both extracts, while isorhyncophylline and rutin were detected only in {ALE}. {ABE}, {ALE} and mitraphylline inhibited the transcription of nuclear factor kappa-{B} in cell cultures, {ALE} and mitraphylline reduced the production of interleukin ({IL}) - 6, and mitraphylline reduced production of tumor necrosis factor-alpha. {T}reatment with {ABE} and {ALE} at 50 and 200 mg kg(1)(-), respectively, reduced respiratory elastance and tissue damping and elastance. {ABE} and {ALE} reduced the number of eosinophils in {BAL}, while {ALE} at 200 mg kg(-1) reduced the levels of {IL}-4 and {IL}-5 in the lung homogenate. {P}eribronchial inflammation was significantly reduced by treatment with {ABE} and {ALE} at 50 and 100 mg kg(-1) respectively. {C}onclusion: {T}he results clarify for the first time the anti-inflammatory activity of {U}. tomentosa in a murine model of asthma. {A}lthough {ABE} and {ALE} exhibited distinct chemical compositions, both extracts inhibited the production of pro-inflammatory cytokines in vitro. {I}n vivo assays revealed that {ABE} was more effective in treating asthmatic inflammation while {ALE} was more successful in controlling respiratory mechanics. {B}oth extracts may have promising applications in the phytotherapy of allergic asthma.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {E}thnopharmacology},
  volume = {218},
  numero = {},
  pages = {76--89},
  ISSN = {0378-8741},
  year = {2018},
  DOI = {10.1016/j.jep.2018.02.013},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075955},
}