@article{fdi:010075729,
  title = {{M}edical countermeasures during the 2018 {E}bola virus disease outbreak in the {N}orth {K}ivu and {I}turi {P}rovinces of the {D}emocratic {R}epublic of the {C}ongo : a rapid genomic assessment},
  author = {{M}bala-{K}ingebeni, {P}. and {A}ziza, {A}. and {D}i {P}aola, {N}. and {W}iley, {M}. {R}. and {M}akiala-{M}andanda, {S}. and {C}aviness, {K}. and {P}ratt, {C}. {B}. and {L}adner, {J}. {T}. and {K}ugelman, {J}. {R}. and {P}rieto, {K}. and {C}hitty, {J}. {A}. and {L}arson, {P}. {A}. and {B}eitzel, {B}. and {A}youba, {A}hidjo and {V}idal, {N}icole and {K}arhemere, {S}. and {D}iop, {M}. and {D}iagne, {M}. {M}. and {F}aye, {M}. and {F}aye, {O}. and {A}runa, {A}. and {N}sio, {J}. and {M}ulangu, {F}. and {M}ukadi, {D}. and {M}ukadi, {P}. and {K}ombe, {J}. and {M}ulumba, {A}. and {V}illabona-{A}renas, {C}. {J}. and {P}ukuta, {E}. and {G}onzalez, {J}. and {B}artlett, {M}. {L}. and {S}ozhamannan, {S}. and {G}ross, {S}. {M}. and {S}chroth, {G}. {P}. and {T}im, {R}. and {Z}hao, {J}. {H}. {J}. and {K}uhn, {J}. {H}. and {D}iallo, {B}. and {Y}ao, {M}. and {F}all, {I}. {S}. and {N}djoloko, {B}. and {M}ossoko, {M}. and {L}acroix, {A}udrey and {D}elaporte, {E}. and {S}anchez-{L}ockhart, {M}. and {S}all, {A}. {A}. and {M}uyembe-{T}amfum, {J}. {J}. and {P}eeterst, {M}. and {P}alacios, {G}. and {A}huka-{M}undeke, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he real-time generation of information about pathogen genomes has become a vital goal for transmission analysis and characterisation in rapid outbreak responses. {I}n response to the recently established genomic capacity in the {D}emocratic {R}epublic of the {C}ongo, we explored the real-time generation of genomic information at the start of the 2018 {E}bola virus disease ({EVD}) outbreak in {N}orth {K}ivu {P}rovince. {M}ethods {W}e used targeted-enrichment sequencing to produce two coding-complete {E}bola virus genomes 5 days after declaration of the {EVD} outbreak in {N}orth {K}ivu. {S}ubsequent sequencing efforts yielded an additional 46 genomes. {G}enomic information was used to assess early transmission, medical countermeasures, and evolution of {E}bola virus. {F}indings {T}he genomic information demonstrated that the {EVD} outbreak in the {N}orth {K}ivu and {I}turi {P}rovinces was distinct from the 2018 {EVD} outbreak in {E}quateur {P}rovince of the {D}emocratic {R}epublic of the {C}ongo. {P}rimer and probe mismatches to {E}bola virus were identified in silico for all deployed diagnostic {PCR} assays, with the exception of the {C}epheid {G}ene{X}pert {GP} assay. {I}nterpretation {T}he first two coding-complete genomes provided actionable information in real-time for the deployment of the r{VSVAG}-{ZEBOV}-{GP} {E}bola virus envelope glycoprotein vaccine, available therapeutics, and sequence-based diagnostic assays. {B}ased on the mutations identified in the {E}bola virus surface glycoprotein ({GP}(1,2)) observed in all 48 genomes, deployed monoclonal antibody therapeutics (m{A}b114 and {ZM}app) should be efficacious against the circulating {E}bola virus variant. {R}apid {E}bola virus genomic characterisation should be included in routine {EVD} outbreak response procedures to ascertain efficacy of medical countermeasures.},
  keywords = {{REPUBLIQE} {DEMOCRATIQUE} {DU} {CONGO}},
  booktitle = {},
  journal = {{L}ancet {I}nfectious {D}iseases},
  volume = {19},
  numero = {6},
  pages = {648--657},
  ISSN = {1473-3099},
  year = {2019},
  DOI = {10.1016/s1473-3099(19)30118-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075729},
}