Bethune Kévin auteur aut IRD Mariac Cédric auteur aut IRD Couderc Marie auteur aut IRD Scarcelli Nora auteur aut IRD Santoni S. auteur aut IRD Ardisson M. auteur aut IRD Martin J. F. auteur aut IRD Montufar R. auteur aut IRD Klein V. auteur aut IRD Sabot François auteur aut IRD Vigouroux Yves auteur aut IRD Couvreur Thomas auteur aut IRD text journalArticle eng text/pdf born digital access Premise Third-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to complete plastid genomes. Methods and Results The protocol uses cost-effective in-house probes developed via long-range PCR and was used in six non-model monocot species (Poaceae: African rice, pearl millet, fonio; and three palm species). DNA was extracted from fresh and silica gel-dried leaves. Our protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from 15% to 98%. DNA extracted from silica gel-dried leaves led to low-quality plastome assemblies when compared to DNA extracted from fresh tissue. Conclusions Our protocol could also be generalized to capture long sequences from specific nuclear fragments. specialized de novo assembly DNA probes long-range PCR MinION whole plastome sequencing 076 020 7 5 e1243 [13 ] 2019 2168-0450 https://www.documentation.ird.fr/hor/fdi:010075713 10.1002/aps3.1243 2168-0450 [F B010075713] https://www.documentation.ird.fr/hor/fdi:010075713 https://www.documentation.ird.fr/intranet/publi/2019/06/010075713.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-07-05 2025-02-24 fdi:010075713 fre