Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Applications in Plant Sciences e1243 [13 ] de novo assembly DNA probes long-range PCR MinION whole plastome sequencing 2019 fdi:010075713 ENG Applications in Plant Sciences 2168-0450 ISI:000468315800007 5 10.1002/aps3.1243 https://www.documentation.ird.fr/hor/fdi:010075713 https://www.documentation.ird.fr/intranet/publi/2019/06/010075713.pdf 7 Horizon (IRD) Premise Third-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to complete plastid genomes. Methods and Results The protocol uses cost-effective in-house probes developed via long-range PCR and was used in six non-model monocot species (Poaceae: African rice, pearl millet, fonio; and three palm species). DNA was extracted from fresh and silica gel-dried leaves. Our protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from 15% to 98%. DNA extracted from silica gel-dried leaves led to low-quality plastome assemblies when compared to DNA extracted from fresh tissue. Conclusions Our protocol could also be generalized to capture long sequences from specific nuclear fragments. 076 ; 020 UR232 / UR022 Équateur