Long-fragment targeted capture for long-read sequencing of plastomes /Bethune, Kévin /Mariac, Cédric /Couderc, Marie /Scarcelli, Nora Santoni, S. Ardisson, M. Martin, J. F. Montufar, R. Klein, V. /Sabot, François /Vigouroux, Yves /Couvreur, Thomas de novo assembly DNA probes long-range PCR MinION whole plastome sequencing Premise Third-generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities for the study of biodiversity, phylogeography, and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to complete plastid genomes. Methods and Results The protocol uses cost-effective in-house probes developed via long-range PCR and was used in six non-model monocot species (Poaceae: African rice, pearl millet, fonio; and three palm species). DNA was extracted from fresh and silica gel-dried leaves. Our protocol successfully captured long-read plastome fragments (3151 bp median on average), with an enrichment rate ranging from 15% to 98%. DNA extracted from silica gel-dried leaves led to low-quality plastome assemblies when compared to DNA extracted from fresh tissue. Conclusions Our protocol could also be generalized to capture long sequences from specific nuclear fragments. 2019 text https://www.documentation.ird.fr/hor/fdi:010075713 fdi:010075713 Bethune Kévin, Mariac Cédric, Couderc Marie, Scarcelli Nora, Santoni S., Ardisson M., Martin J. F., Montufar R., Klein V., Sabot François, Vigouroux Yves, Couvreur Thomas. Long-fragment targeted capture for long-read sequencing of plastomes. 2019, 7 (5), e1243 [13 ] EN