Gonzalez Bautista E. auteur aut IRD Gutierrez E. auteur aut IRD Dupuy N. auteur aut IRD Perraud Gaime Isabelle auteur aut IRD Ziarelli F. auteur aut IRD Farnet da Silva A. M. auteur aut IRD text journalArticle eng text/pdf born digital access Production of second-generation bioethanol uses lignocellulose from agricultural by-products such as sugarcane bagasse (SCB). A lignocellulose pre-treatment is required to degrade lignin, ensuring further efficient saccharification. Two experimental designs were set up to define culture conditions of Pycnoporus sanguineus in mesocosms to increase laccase activities and thus delignification. The first experimental design tested the effect of phenolic complementation (via coffee pulp) and the use of urea as a simple nitrogen source and the second defined more precisely the percentages of coffee pulp and urea to enhance delignification. The responses measured were: lignocellulolytic activities, laccase isoform profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the chemical transformation of the substrate using solid-state NMR of 13C. Adding 10% of coffee pulp increased laccase activities and fungal biomass (32.5% and 16% respectively), enhanced two constitutive isoforms (R-f 0.23 and 0.27), induced a new isoform (R-f 0.19) and led to a decrease in total aromatics. However, higher concentrations of coffee pulp (25%) decreased laccase and cellulase activities but no decrease in aromaticity was observed, potentially due to the toxic effect of phenols from coffee pulp. Moreover, laccase production was still inhibited even for lower concentrations of urea (0-5%). Our findings revealed that an agricultural by-product like coffee pulp can enhance laccase activity-though to a threshold- and that urea limited this process, indicating that other N-sources should be tested for the biological delignification of SCB. specialized Dephenolisation laccase induction Second-generation bioethanol Solid-state fermentation White-rot fungi 084 239 178-186 2019 0301-4797 https://www.documentation.ird.fr/hor/fdi:010075704 10.1016/j.jenvman.2019.03.033 0301-4797 [F B010075704] https://www.documentation.ird.fr/hor/fdi:010075704 https://www.documentation.ird.fr/intranet/publi/2019/06/010075704.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-07-05 2025-02-24 fdi:010075704 fre