@article{fdi:010075704,
  title = {{P}re-treatment of a sugarcane bagasse-based substrate prior to saccharification : effect of coffee pulp and urea on laccase and cellulase activities of {P}ycnoporus sanguineus},
  author = {{G}onzalez {B}autista, {E}. and {G}utierrez, {E}. and {D}upuy, {N}. and {P}erraud {G}aime, {I}sabelle and {Z}iarelli, {F}. and {F}arnet da {S}ilva, {A}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}roduction of second-generation bioethanol uses lignocellulose from agricultural by-products such as sugarcane bagasse ({SCB}). {A} lignocellulose pre-treatment is required to degrade lignin, ensuring further efficient saccharification. {T}wo experimental designs were set up to define culture conditions of {P}ycnoporus sanguineus in mesocosms to increase laccase activities and thus delignification. {T}he first experimental design tested the effect of phenolic complementation (via coffee pulp) and the use of urea as a simple nitrogen source and the second defined more precisely the percentages of coffee pulp and urea to enhance delignification. {T}he responses measured were: lignocellulolytic activities, laccase isoform profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ({SDS}-{PAGE}) and the chemical transformation of the substrate using solid-state {NMR} of 13{C}. {A}dding 10% of coffee pulp increased laccase activities and fungal biomass (32.5% and 16% respectively), enhanced two constitutive isoforms ({R}-f 0.23 and 0.27), induced a new isoform ({R}-f 0.19) and led to a decrease in total aromatics. {H}owever, higher concentrations of coffee pulp (25%) decreased laccase and cellulase activities but no decrease in aromaticity was observed, potentially due to the toxic effect of phenols from coffee pulp. {M}oreover, laccase production was still inhibited even for lower concentrations of urea (0-5%). {O}ur findings revealed that an agricultural by-product like coffee pulp can enhance laccase activity-though to a threshold- and that urea limited this process, indicating that other {N}-sources should be tested for the biological delignification of {SCB}.},
  keywords = {{D}ephenolisation ; laccase induction ; {S}econd-generation bioethanol ; {S}olid-state fermentation ; {W}hite-rot fungi},
  booktitle = {},
  journal = {{J}ournal of {E}nvironmental {M}anagement},
  volume = {239},
  numero = {},
  pages = {178--186},
  ISSN = {0301-4797},
  year = {2019},
  DOI = {10.1016/j.jenvman.2019.03.033},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075704},
}