Ayala Diego auteur aut IRD Zhang S. auteur aut IRD Chateau M. auteur aut IRD Fouet C. auteur aut IRD Morlais Isabelle auteur aut IRD Costantini Carlo auteur aut IRD Hahn M. W. auteur aut IRD Besansky N. J. auteur aut IRD text journalArticle eng text/pdf born digital access Inversion polymorphisms are responsible for many ecologically important phenotypes and are often found under balancing selection. However, the same features that ensure their large role in local adaptation-especially reduced recombination between alternate arrangements-mean that uncovering the precise loci within inversions that control these phenotypes is unachievable using standard mapping approaches. Here, we take advantage of long-term balancing selection on a pair of inversions in the mosquito Anopheles gambiae to map desiccation tolerance via pool-GWAS. Two polymorphic inversions on chromosome 2 of this species (denoted 2La and 2Rb) are associated with arid and hot conditions in Africa and are maintained in spatially and temporally heterogeneous environments. After measuring thousands of wild-caught individuals for survival under desiccation stress, we used phenotypically extreme individuals homozygous for alternative arrangements at the 2La inversion to construct pools for whole-genome sequencing. Genomewide association mapping using these pools revealed dozens of significant SNPs within both 2La and 2Rb, many of which neighboured genes controlling ion channels or related functions. Our results point to the promise of similar approaches in systems with inversions maintained by balancing selection and provide a list of candidate genes underlying the specific phenotypes controlled by the two inversions studied here. specialized aridity tolerance balancing selection local adaptation recombination CAMEROUN 052 020 28 6 1333-1342 2019 0962-1083 https://www.documentation.ird.fr/hor/fdi:010075644 10.1111/mec.14880 0962-1083 [F B010075644] https://www.documentation.ird.fr/hor/fdi:010075644 https://www.documentation.ird.fr/intranet/publi/2019/05/010075644.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-06-04 2025-02-24 fdi:010075644 fre