@article{fdi:010075611, title = {{EST}-{SSR} development for {D}igitaria exilis and its relatives {D}. iburua and {D}. longiflora from transcriptome sequences}, author = {{O}lodo, {K}. {F}. and {G}ueye, {M}. {C}. and {C}alatayud, {C}. and {D}iop, {B}. {M}. and {K}ane, {N}. {A}. and {N}gom, {A}. and {N}tui, {V}. {O}. and {B}arreto, {M}. {M}. {S}. and {U}yoh, {E}. {A}. and {A}braham, {S}. and {V}igouroux, {Y}ves and {B}illot, {C}. and {B}arnaud, {A}deline}, editor = {}, language = {{ENG}}, abstract = {{D}igitaria exilis is an important indigenous cereal in {W}est {A}frica. {T}he first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. {A} total of 126 new putative primer pairs were successfully designed in 37,327 unigenes from the {D}. exilis transcriptome. {T}hirty-seven primer pairs were randomly selected and tested for their ability to cross-amplify to related species. {C}lear amplification patterns were observed on 24 primer pairs. {O}f these, 71, 74 and 35% showed polymorphism in three species: {D}. exilis, {D}. longiflora and {D}. iburua. {T}he transferability from {D}. exilis was 96% to {D}. longiflora and 71% to {D}. iburua. {T}he new {SSR} markers confirmed the close genetic proximity of {D}. exilis with {D}. longiflora and its stronger genetic difference of {D}. exilis from {D}. iburua. {T}hese markers will be valuable for completing future knowledge on {D}igitaria evolutionary history, and for testing gene flows between related species.}, keywords = {cross-amplification ; {D}igitaria ; diversity ; {EST}-{SSR} ; microsatellite ; {P}oaceae ; {AFRIQUE} {DE} {L}'{OUEST} ; {BENIN} ; {BURKINA} {FASO} ; {MALI} ; {GUINEA} ; {NIGER} ; {SENEGAL} ; {TOGO} ; {NIGERIA} ; {SENEGAL}}, booktitle = {}, journal = {{P}lant {G}enetic {R}esources}, volume = {17}, numero = {3}, pages = {280--284}, ISSN = {1479-2621}, year = {2019}, DOI = {10.1017/s1479262118000400}, URL = {https://www.documentation.ird.fr/hor/fdi:010075611}, }