Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Virology 75-84 Cryo-EM Three-dimensional structure Single-particle analysis BBSV Comovirus Virus coat protein Single-stranded RNA genome 2019 fdi:010075598 ENG Virology 0042-6822 ISI:000464770400010 10.1016/j.virol.2019.02.009 https://www.documentation.ird.fr/hor/fdi:010075598 https://www.documentation.ird.fr/intranet/publi/2019/05/010075598.pdf 530 Horizon (IRD) The Broad bean stain virus (BBSV) is a member of the genus Comovirus infecting Fabaceae. The virus is transmitted through seed and by plant weevils causing severe and widespread disease worldwide. BBSV has a bipartite, positive-sense, single-stranded RNA genome encapsidated in icosahedral particles. We present here the cryo-electron microscopy reconstruction of the BBSV and an atomic model of the capsid proteins refined at 3.22 angstrom resolution. We identified residues involved in RNA/capsid interactions revealing a unique RNA genome organization. Inspection of the small coat protein C-terminal domain highlights a maturation cleavage between Leu567 and Leu568 and interactions of the C-terminal stretch with neighbouring small coat proteins within the capsid pentameric turrets. These interactions previously proposed to play a key role in the assembly of the Cowpea mosaic virus suggest a common capsid assembly mechanism throughout all comovirus species. 076 ; 020 UR186