Gomez-Santos N. auteur aut IRD Glatter T. auteur aut IRD Koebnik Ralf auteur aut IRD Swiatek-Polatynska M. A. auteur aut IRD Sogaard-Andersen L. auteur aut IRD text journalArticle eng text/pdf reformatted digital access TonB-dependent transporters (TBDTs) are ubiquitous outer membrane beta-barrel proteins that import nutrients and bacteriocins across the outer membrane in a proton motive force-dependent manner, by directly connecting to the ExbB/ExbD/TonB system in the inner membrane. Here, we show that the TBDT Oar in Myxococcus xanthus is required for secretion of a protein, protease PopC, to the extracellular milieu. PopC accumulates in the periplasm before secretion across the outer membrane, and the proton motive force has a role in secretion to the extracellular milieu. Reconstitution experiments in Escherichia coli demonstrate that secretion of PopC across the outer membrane not only depends on Oar but also on the ExbB/ExbD/TonB system. Our results indicate that TBDTs and the ExbB/ExbD/TonB system may have roles not only in import processes but also in secretion of proteins. specialized 020 10 1360 [18 ] 2019 2041-1723 https://www.documentation.ird.fr/hor/fdi:010075515 10.1038/s41467-019-09366-9 2041-1723 [F B010075515] https://www.documentation.ird.fr/hor/fdi:010075515 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-04/010075515.pdf IRD - Base Horizon / Pleins textes 2019-05-13 2025-02-24 fdi:010075515 fre