@article{fdi:010075498,
  title = {{C}omparative analysis of two sister {E}rythrophleum species ({L}eguminosae) reveal contrasting transcriptome-wide responses to early drought stress},
  author = {{N}eji, {M}. and {G}orel, {A}. and {O}jeda, {D}. {I}. and {D}uminil, {J}{\'e}r{\^o}me and {K}astally, {C}. and {S}teppe, {K}. and {F}ayolle, {A}. and {H}ardy, {O}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}ith the ongoing climate change, {A}frican rainforests are expected to experience severe drought events in the future. {I}n {A}frica, the tropical genus {E}rythrophleum ({F}abaceae) includes two forest sister timber tree species displaying contrasting geographical distributions. {E}rythrophleum ivorense is adapted to wet evergreen {G}uineo-{C}ongolian forests, whereas {E}. suaveolens occurs in a wider range of climates, being found in moist dense forests but also in gallery forests under a relatively drier climate. {T}his geographical distribution pattern suggests that the two species might cope differently to drought at the genomic level. {Y}et, the genetic basis of tolerance response to drought stress in both species is still uncharacterized. {T}o bridge this gap, we performed an {RNA}-seq approach on seedlings from each species to monitor their transcriptional responses at different levels of drought stress (0, 2 and 6 weeks after stopping watering seedlings). {M}onitoring of wilting symptoms revealed that {E}. suaveolens displayed an earlier phenotypic response to drought stress than {E}. ivorense. {A}t the transcriptomic level, results revealed 2020 (1204 down-regulated/816 up-regulated) and 1495 differentially expressed genes ({DEG}s) in response to drought stress from a total of 67,432 and 66,605 contigs assembled in {E}. ivorense and {E}. suaveolens, respectively. {A}fter identifying 30,374 orthologs between species, we found that only 7 of them were {DEG}s shared between species, while 587 and 458 were differentially expressed only in {E}. ivorense or {E}. suaveolens, respectively. {GO} and {KEGG} enrichment analysis revealed that the two species differ in terms of significantly regulated pathways as well as the number and expression profile of {DEG}s ({U}p/{D}own) associated with each pathway in the two stress stages. {O}ur results suggested that the two studied species react differently to drought. {E}. suaveolens seems displaying a prompt response to drought at its early stage strengthened by the down-regulation of many {DEG}s encoding for signaling and metabolism-related pathways. {A} considerable up-regulation of these pathways was also found in {E}. ivorense at the late stage of drought, suggesting this species may be a late responder. {O}verall, our data may serve as basis for further understanding the genetic control of drought tolerance in tropical trees and favor the selection of crucial genes for genetically enhancing drought resistance.},
  keywords = {{A}frican rainforests ; {E}rythrophleum ; {RNA}-seq ; {T}ranscriptomic ; {D}ifferentially expressed genes ; {D}rought tolerance},
  booktitle = {},
  journal = {{G}ene},
  volume = {694},
  numero = {},
  pages = {50--62},
  ISSN = {0378-1119},
  year = {2019},
  DOI = {10.1016/j.gene.2019.01.027},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075498},
}