@article{fdi:010075346,
  title = {{A}ntigenic specificity of the 72-kilodalton major surface glycoprotein of {L}eishmania braziliensis braziliensis},
  author = {{K}utner, {S}. and {P}ellerin, {P}. and {B}reni{\`e}re {S}imone {F}r{\'e}d{\'e}rique and {D}esjeux, {P}. and {D}edet, {J}.{P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e examined the expression and the antigenicity of the major surface polypeptides of {L}eishmania braziliensis braziliensis and {L}eishmania donovani chagasi, parasites which commonly coexist in the same endemic areas of {B}olivia. {S}odium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles from surface-iodinated promastigotes showed the presence of a unique iodinatable polypeptide of 72 k{D}a on the {L}. b. braziliensis surface and of two major components of 65 and 50 k{D}a exposed at the surface of {L}. d. chagasi. {C}omparison of the peptide digestion profiles of the major iodinated polypeptides of both strains showed no similarity between the maps of the 72- and the 65-k{D}a polypeptides of {L}. b. braziliensis and {L}. d. chagasi, respectively. {I}mmunoprecipitation of surface-labeled {L}. b. braziliensis {N}onidet {P}-40 extracts with 35 serum specimens obtained from {B}olivian patients with cutaneous and mucocutaneous leishmaniasis showed that all serum specimens recognized predominantly the 72-k{D}a antigen and high-molecular-mass proteins in some cases. {T}he recognition patterns were independent of the geographical origin of the patient, the type of lesion, and the serum antibody titer. {S}erum specimens from children with visceral leishmaniasis did not precipitate the {L}. b. braziliensis 72-k{D}a antigen. {H}amster hyperimmune serum against {L}. b. braziliensis also recognized the 72-k{D}a surface antigen. {H}owever, this recognition was inhibited in the presence of the homologous nonlabeled antigen but not in the presence of heterologous ({L}. d. chagasi and {T}rypanosoma cruzi) antigens. {T}he specific recognition of the 72-k{D}a surface antigen in both natural and experimental {L}. b. braziliensis infections suggests that this antigen could be a good candidate for use in the differential immunodiagnosis and prognosis of the disease.},
  keywords = {{TRYPANOSOMIASE} {HUMAINE} ; {MALADIE} {DE} {CHAGAS} ; {BOLIVIE}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {29},
  numero = {3},
  pages = {595--599},
  ISSN = {0095-1137},
  year = {1991},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075346},
}