@article{fdi:010075275, title = {{R}egulation of {FUSCA}3 expression during seed development in {A}rabidopsis}, author = {{R}oscoe, {T}. {J}. and {V}aissayre, {V}irginie and {P}aszkiewicz, {G}. and {C}lavijo, {F}. and {K}elemen, {Z}. and {M}ichaud, {C}aroline and {L}epiniec, {L}. and {D}ubreucq, {B}. and {Z}hou, {D}. {X}. and {D}evic, {M}.}, editor = {}, language = {{ENG}}, abstract = {{FUSCA}3 ({FUS}3) is a master regulator of seed development important in establishing and maintaining embryonic identity whose expression is tightly regulated at genetic and epigenetic levels. {D}espite this prominent role, the control of {FUS}3 expression remains poorly understood. {P}romoter and functional complementation analyses provided insight into the regulation of {FUS}3. {W}-boxes present in the promoter proximal to the start of transcription are recognized by {WRKY} type-1 factors which are necessary for the activation of {FUS}3 expression. {T}he {RY} motif, the binding site of {B}3 factors, is important for the activation of {FUS}3 in the embryo proper but not in the suspensor. {T}he loss of a negative regulatory sequence ({NRS}) leads to preferential expression of {FUS}3 in the vasculature of vegetative tissues. {S}ince the {NRS} includes the {RY} motif, mechanisms of activation and repression target adjacent or overlapping regions. {T}hese findings discriminate the regulation of {FUS}3 from that of {LEAFY} {COTYLEDON}2 by the control exerted by {WRKY} factors and by the presence of the {RY} motif, yet also confirm conservation of certain regulatory elements, thereby implicating potential regulation by {BASIC} {PENTACYSTEINE} ({BPC}) factors and {POLYCOMB} {REPRESSIVE} {COMPLEX}2 ({PRC}2).}, keywords = {{A}rabidopsis thaliana ; {FUSCA}3 ; {R}egulation of expression ; {S}eed maturation ; {S}eed reserves ; {WRKY}2}, booktitle = {}, journal = {{P}lant and {C}ell {P}hysiology}, volume = {60}, numero = {2}, pages = {476--487}, ISSN = {0032-0781}, year = {2019}, DOI = {10.1093/pcp/pcy224}, URL = {https://www.documentation.ird.fr/hor/fdi:010075275}, }