@article{fdi:010075263,
  title = {{H}igh prevalence and characterization of extended-spectrum ss-lactamase producing {E}nterobacteriaceae in {C}hadian hospitals},
  author = {{M}ahamat, {O}. {O}. and {L}ounnas, {M}anon and {H}ide, {M}. and {D}umont, {Y}. and {T}idjani, {A}. and {K}amougam, {K}. and {A}bderrahmane, {M}. and {B}enavides, {J}. and {S}olassol, {J}. and {B}anuls, {A}nne-{L}aure and {J}ean-{P}ierre, {H}. and {C}arriere, {C}. and {G}odreuil, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground{E}xtended-spectrum ss-lactamase-producing {E}nterobacteriaceae ({ESBL}-{PE}) represent a major problem in the management of nosocomial infections. {H}owever, {ESBL}-{PE} are not systematically monitored in {A}frican countries. {T}he aim of this study was to determine {ESBL}-{PE} prevalence in patients from three hospitals in {N}'{D}jamena, the capital city of {C}had, and to characterize the genetic origin of the observed resistance.{M}ethods{F}rom {J}anuary to {M}arch 2017, 313 non-duplicate isolates were recovered from various clinical specimens obtained from 1713 patients in the three main hospitals of {N}'{D}jamena. {B}acterial species were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. {S}usceptibility to 28 antibiotics was tested using the disk diffusion method on {M}uller-{H}inton agar, and {ESBL} production was confirmed with the double-disc synergy test. {T}he most prevalent {ESBL} genes associated with the observed resistance were detected using multiplex {PCR} followed by double-stranded {DNA} sequencing.{R}esults{A}mong the 313 isolates, 197 belonged to the {E}nterobacteriaceae family. {T}he overall {ESBL}-{PE} prevalence was 47.72% (n=94/197), with a higher rate among inpatients compared with outpatients (54.13% vs. 34.37%). {ESBL}-{PE} prevalence was highest in older patients (60years of age). {E}. coli was the most common {ESBL}-producer organism (63.8%), followed by {K}. pneumoniae (21.2%). {ESBL}-{PE} were mainly found in urine samples (75%). {T}he {CTX}-{M}-1 group was dominant (96.7% of the 94 {ESBL}-{PE} isolates, {CTX}-{M}-15 enzyme), followed by the {CTX}-{M}-9 group (4.1%). 86% of resistant isolates harbored more than one {ESBL}-encoding gene. {ESBL} production was also associated with the highest levels of resistance to non--lactam drugs.{C}onclusions{T}he prevalence of {ESBL}-{PE} harboring resistant genes encoding {ESBL}s of the {CTX}-{M}-1 group was high (48%) among clinical isolates of three main hospitals in {C}had, suggesting an alarming spread of {ESBL}-{PE} among patients.},
  keywords = {{ESBL} ; {E}nterobacteriaceae ; {C}linical samples ; {P}revalence ; {C}had ; {TCHAD}},
  booktitle = {},
  journal = {{BMC} {I}nfectious {D}iseases},
  volume = {19},
  numero = {},
  pages = {art. 205 [7 p.]},
  ISSN = {1471-2334},
  year = {2019},
  DOI = {10.1186/s12879-019-3838-1},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075263},
}