@article{fdi:010075261, title = {{O}ntogeny of the digestive enzyme activity of the {A}mazonian pimelodid catfish {P}seudoplatystoma punctifer ({C}astelnau, 1855)}, author = {{C}astro-{R}uiz, {D}. and {M}ozanzadeh, {M}. {T}. and {F}ernandez-{M}endez, {C}. and {A}ndree, {K}. {B}. and {G}arcia-{D}avila, {C}. and {C}ahu, {C}. and {G}isbere, {E}. and {D}arias, {M}aria {J}ose}, editor = {}, language = {{ENG}}, abstract = {{T}he aim of the study was to evaluate the functional ontogeny of the digestive system of {P}seudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase {N}, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. {L}arvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae {L}-1 , 27.8 +/- 0.7 degrees {C} and 0 {L}: 24{D} photoperiod. {L}arvae were fed from 4 to 17 dpf with {A}nemia nauplii and weaned onto an experimentally formulated feed (crude protein content similar to 45%; crude fat content similar to 10%; crude carbohydrate similar to 8%) within 3 days, then continued with the same diet until the end of the trial. {P}. punctifer showed an exponential growth pattern with two different growth rates: a slower one from hatching to 12 dpf followed by a faster one from 12 to 27 dpf. {T}he specific and total activities of the pancreatic and intestinal enzymes were detected from hatching. {T}he digestive system was functional at 12 dpf, indicating the transition from the larval to the juvenile stage (alkaline to acid digestion). {T}herefore individuals could be weaned from that day onwards. {T}he variations observed in the enzymatic activity from 17 dpf reflected the adaptation of the enzymatic machinery to the new diet supplied. {P}. punctifer larvae showed a fast digestive system development with an enzymatic profile typical of a tropical and carnivorous species.}, keywords = {{P}seudoplatystoma punctifer ; {O}ntogeny ; {E}nzymatic activity ; {D}igestive system ; {B}rush border ; {P}ancreatic enzymes ; {G}astric enzymes ; {PEROU} ; {AMAZONIE}}, booktitle = {}, journal = {{A}quaculture}, volume = {504}, numero = {}, pages = {210--218}, ISSN = {0044-8486}, year = {2019}, DOI = {10.1016/j.aquaculture.2019.01.059}, URL = {https://www.documentation.ird.fr/hor/fdi:010075261}, }