@article{fdi:010075198,
  title = {{G}enome-wide profiling of human papillomavirus {DNA} integration in liquid-based cytology specimens from a {G}abonese female population using {HPV} capture technology},
  author = {{N}kili-{M}eyong, {A}. {A}. and {M}oussavou-{B}oundzanga, {P}. and {L}abouba, {I}. and {K}oumakpayi, {I}. {H}. and {J}eannot, {E}. and {D}escorps-{D}eclere, {S}. and {S}astre-{G}arau, {X}. and {L}eroy, {E}ric and {B}elembaogo, {E}. and {B}erthet, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}uman papillomavirus ({HPV}) is recognised as the cause of precancerous and cancerous cervical lesions. {F}urthermore, in high-grade lesions, {HPV} is frequently integrated in the host cell genome and associated with the partial or complete loss of the {E}1 and {E}2 genes, which regulate the activity of viral oncoproteins {E}6 and {E}7. {I}n this study, using a double-capture system followed by high-throughput sequencing, we determined the {HPV} integration status present in liquid-based cervical smears in an urban {G}abonese population. {T}he main inclusion criteria were based on cytological grade and the detection of the {HPV}16 genotype using molecular assays. {T}he rate of {HPV} integration in the host genome varied with cytological grade: 85.7% (6/7), 71.4% (5/7), 66.7% (2/3) 60% (3/5) and 30.8% (4/13) for carcinomas, {HSIL}, {ASCH}, {LSIL} and {ASCUS}, respectively. {F}or high cytological grades (carcinomas and {HSIL}), genotypes {HPV}16 and 18 represented 92.9% of the samples (13/14). {T}he integrated form of {HPV}16 genotype was mainly found in high-grade lesions in 71.4% of samples regardless of cytological grade. {M}inority genotypes ({HPV}33, 51, 58 and 59) were found in {LSIL} samples, except {HPV}59, which was identified in one {HSIL} sample. {A}mong all the {HPV} genotypes identified after double capture, 10 genotypes ({HPV}30, 35, 39, 44, 45, 53, 56, 59, 74 and 82) were detected only in episomal form. {O}ur study revealed that the degree of {HPV} integration varies with cervical cytological grade. {T}he integration event might be a potential clinical prognostic biomarker for the prediction of the progression of neoplastic lesions.},
  keywords = {{GABON}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {9},
  numero = {},
  pages = {art. 1504 [11 p.]},
  ISSN = {2045-2322},
  year = {2019},
  DOI = {10.1038/s41598-018-37871-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010075198},
}