Thiodiketopiperazines with two spirocyclic centers extracted from Botryosphaeria mamane, an endophytic fungus isolated from Bixa orellana l Barakat, F. Vansteelandt, M. Triastuti, A. Jargeat, P. Jacquemin, D. Graton, J. Mejia, K. Cabanillas, B. Vendier, L. Stigliani, J. L. /Haddad, Mohamed Fabre, N. Botryosphaeria mamane DE Gardner (Botryosphaeriaceae) Bixa orellana L. (Bixaceae) Thiodiketopiperazine Cytotoxic activity Three thiodiketopiperazines, botryosulfuranols A-C (1-3) were isolated from the endophytic fungus Botryosphaeria mamane. The three compounds present sulfur atoms on alpha- and beta-positions of phenylalanine derived residues and unprecedented two spirocyclic centers at C-4 and C-2'. Their planar structures were determined by spectroscopic analysis and absolute configurations were achieved by X-ray diffraction analysis and ECD and NMR chemical shifts calculations. Botryosulfuranol A (1) was the most cytotoxic compound against four cancer cell lines (HT-29, HepG2, Caco-2, HeLa) and two healthy cell lines (IEC6, Vero) highlighting the importance of an electrophilic center for cell growth inhibition. 2019 text https://www.documentation.ird.fr/hor/fdi:010074948 fdi:010074948 Barakat F., Vansteelandt M., Triastuti A., Jargeat P., Jacquemin D., Graton J., Mejia K., Cabanillas B., Vendier L., Stigliani J. L., Haddad Mohamed, Fabre N.. Thiodiketopiperazines with two spirocyclic centers extracted from Botryosphaeria mamane, an endophytic fungus isolated from Bixa orellana l. 2019, 158, 142-148 EN PEROU