Tao D. Y. auteur aut IRD McGill B. auteur aut IRD Hamerly T. auteur aut IRD Kobayashi T. auteur aut IRD Khare P. auteur aut IRD Dziedzic A. auteur aut IRD Leski T. auteur aut IRD Holtz A. auteur aut IRD Shull B. auteur aut IRD Jedlicka A. E. auteur aut IRD Walzer A. auteur aut IRD Slowey P. D. auteur aut IRD Slowey C. C. auteur aut IRD Nsango S. E. auteur aut IRD Stenger D. A. auteur aut IRD Chaponda M. auteur aut IRD Mulenga M. auteur aut IRD Jacobsen K. H. auteur aut IRD Sullivan D. J. auteur aut IRD Ryan S. J. auteur aut IRD Ansumana R. auteur aut IRD Moss W. J. auteur aut IRD Morlais Isabelle auteur aut IRD Dinglasan R. R. auteur aut IRD text journalArticle eng text/pdf born digital access A large proportion of ongoing malaria parasite transmission is attributed to low-density subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium fakiparum gametocyte carriage is subclinical, but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point-of-need (PON) RDTs for gametocyte detection exist, much less one that can perform noninvasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite markers from which we selected a single candidate for use in a PON RDT. We performed a cross-sectional, multi-omics study of saliva from 364 children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow immunoassay test for P. fakiparum gametocyte carriers. The test is capable of identifying submicroscopic carriage in both clinical and nonclinical settings and is compatible with archived saliva samples. specialized AFRIQUE SUBSAHARIENNE 052 050 11 473 art. eaan4479 [13 ] 2019 1946-6234 https://www.documentation.ird.fr/hor/fdi:010074860 10.1126/scitranslmed.aan4479 1946-6234 [F B010074860] https://www.documentation.ird.fr/hor/fdi:010074860 https://www.documentation.ird.fr/intranet/publi/2019/01/010074860.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-02-04 2025-02-24 fdi:010074860 fre