Tran T. T. auteur aut IRD Doucoure H. auteur aut IRD Hutin M. auteur aut IRD Nino L. M. J. auteur aut IRD Szurek Boris auteur aut IRD Cunnac Sébastien auteur aut IRD Koebnik Ralf auteur aut IRD text journalArticle eng text/pdf reformatted digital access Many plant-pathogenic xanthomonads use a type III secretion system to translocate Transcription Activator-Like (TAL) effectors into eukaryotic host cells where they act as transcription factors. Target genes are induced upon binding of a TAL effector to double-stranded DNA in a sequence-specific manner. DNA binding is governed by a highly repetitive protein domain, which consists of an array of nearly identical repeats of ca. 102 base pairs. Many species and pathovars of Xanthomonas, including pathogens of rice, cereals, cassava, citrus and cotton, encode multiple TAL effectors in their genomes. Some of the TAL effectors have been shown to act as key pathogenicity factors, which induce the expression of susceptibility genes to the benefit of the pathogen. However, due to the repetitive character and the presence of multiple gene copies, high-throughput cloning of TAL effector genes remains a challenge. In order to isolate complete TAL effector gene repertoires, we developed an enrichment cloning strategy based on genome-informed in silico optimization of restriction digestions, selective restriction digestion of genomic DNA, and size fractionation of DNA fragments. Our rapid, cheap and powerful method allows efficient cloning of TAL effector genes from xanthomonads, as demonstrated for two rice-pathogenic strains of Xanthomonas oryzae from Africa. specialized Enrichment cloning Type III secretion Type III effector TAL effector TALome Xanthomonas Bacterial leaf blight Bacterial leaf streak Rice 076 020 5 1027-1032 2018 2215-0161 https://www.documentation.ird.fr/hor/fdi:010074855 10.1016/j.mex.2018.08.014 2215-0161 [F B010074855] https://www.documentation.ird.fr/hor/fdi:010074855 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-01/010074855.pdf IRD - Base Horizon / Pleins textes 2019-02-04 2023-07-11 fdi:010074855 fre