@article{fdi:010074847,
  title = {{A}n integrated analysis of m{RNA} and s{RNA} transcriptional profiles in {C}offea arabica {L}. roots: insights on nitrogen starvation responses},
  author = {dos {S}antos, {T}. {B}. and {S}oares, {J}. {D}. {M}. and {L}ima, {J}. {E}. and {S}ilva, {J}. {C}. and {I}vamoto, {S}. {T}. and {B}aba, {V}. {Y}. and {S}ouza, {S}. {G}. {H}. and {L}orenzetti, {A}. {P}. {R}. and {P}aschoal, {A}. {R}. and {M}eda, {A}. {R}. and {N}ishiyama, {M}. {Y}. and de {O}liveira, {U}. {C}. and {M}okochinski, {J}. {B}. and {G}uyot, {R}omain and {J}unqueira-de-{A}zevedo, {I}. {L}. {M}. and {F}igueira, {A}. {V}. {O}. and {M}azzafera, {P}. and {J}unior, {O}. {R}. and {V}ieira, {L}. {G}. {E}. and {P}ereira, {L}. {F}. {P}. and {D}omingues, {D}. {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}offea arabica {L}. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. {N}itrogen ({N}) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to {N} limitation remain largely unknown in tropical woody crops. {I}n this study, we investigated the transcriptome of coffee roots under {N} starvation, analyzing poly-{A}+ libraries and small {RNA}s. {W}e also evaluated the concentration of selected amino acids and {N}-source preferences in roots. {A}mmonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots. {W}e obtained 34,654 assembled contigs by m{RNA} sequencing, and validated the transcriptional profile of 12 genes by {RT}-q{PCR}. {I}llumina small {RNA} sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 micro{RNA} families targeting 253 genes. {T}he transcriptional pattern of eight mi{RNA} families was also validated. {T}o our knowledge, this is the first catalog of differentially regulated amino acids, {N} sources, m{RNA}s, and s{RNA}s in {A}rabica coffee roots.},
  keywords = {{C}offee ; {N}itrogen transport ; {D}ifferential gene expression ; {RNA}-seq ; micro{RNA}},
  booktitle = {},
  journal = {{F}unctional and {I}ntegrative {G}enomics},
  volume = {19},
  numero = {1},
  pages = {151--169},
  ISSN = {1438-793{X}},
  year = {2019},
  DOI = {10.1007/s10142-018-0634-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074847},
}