Segeral O. auteur aut IRD N'Diaye D. S. auteur aut IRD Prak S. auteur aut IRD Nouhin J. auteur aut IRD Chhun S. auteur aut IRD Khamduang W. auteur aut IRD Chim K. auteur aut IRD Roque-Afonso A. M. auteur aut IRD Piola P. auteur aut IRD Borand L. auteur aut IRD Ngo-Giang-Huong Nicole auteur aut IRD Rouet F. auteur aut IRD ANRS Study Group auteur aut IRD text journalArticle eng text/pdf born digital access Background: In Cambodia, access to hepatitis B surface antigen (HBsAg) screening is low for pregnant women and Hepatitis B Virus (HBV) DNA quantification is poorly accessible. Objectives: To evaluate the performance of a serial algorithm using two HBV rapid diagnostic tests (RDTs), in which samples positive for HBsAg were further tested for HBeAg as a surrogate marker for HBV DNA quantification. Study design: In 2015, we prospectively collected plasma samples from 250 pregnant women consulting for antenatal care in one hospital in Phnom Penh including 128 with a known positive HBsAg status. All specimens were tested with the SD BIOLINE HBsAg RDT and HBsAg ELISA assay. In ELISA-positive samples, HBeAg status was determined using the SD BIOLINE HBeAg RDT and HBV DNA quantification was assessed. Results: Sensitivity and specificity of HBsAg RDT were 99.2% (97.7-99.9) and 100% (97.0-100), respectively. Among the 128 ELISA-positive samples, 29 (23%) tested HBeAg positive and 34 (26.5%) had HBV DNA > 5.3 Log(10) IU/mL. Sensitivity and specificity of HBeAg RDT in identifying viremic samples were 76.5% (62.2.0-90.7) and 96.8% (93.3-100) for HBV DNA > 5.3 Log(10) IU/mL and 89.3% (77.8-100) and 96.0% (92.2-99.8) for HBV DNA > 7.3 Log(10) IU/mL. Among the 99 negative HBeAg RDT women, 8 had HBV DNA > 5.3 Log10 IU/mL and 7 of them harbored BCP/PC HBV mutants. Conclusions: A combination of HBsAg and HBeAg RDTs could be a low-cost strategy to identify HBV-infected pregnant women at risk of perinatal transmission in a country were HBV DNA quantification is not routinely available. specialized Cambodia Hepatitis B virus Rapid diagnostic tests Pregnant women Mother-to-child transmission CAMBODGE 052 050 109 29-34 2018 1386-6532 https://www.documentation.ird.fr/hor/fdi:010074825 10.1016/j.jcv.2018.10.007 1386-6532 [F B010074825] https://www.documentation.ird.fr/hor/fdi:010074825 https://www.documentation.ird.fr/intranet/publi/2019/01/010074825.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2019-02-04 2019-02-08 fdi:010074825 fre