%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sonon, P.
%A Sadissou, I.
%A Tokplonou, L.
%A M'po, K. K. G.
%A Glitho, S. S. C.
%A Agniwo, P.
%A Ibikounle, M.
%A Massaro, J. D.
%A Massougbodji, A.
%A Moreau, P.
%A Sabbagh, A.
%A Mendes, C. T.
%A Moutairou, K. A.
%A Castelli, E. C.
%A Courtin, David
%A Donadi, E. A.
%T HLA-G, -E and -F regulatory and coding region variability and haplotypes in the Beninese Toffin population sample
%D 2018
%L fdi:010074796
%G ENG
%J Molecular Immunology
%@ 0161-5890
%K HLA-E ; HLA-F ; HLA-G ; Haplotypes ; Massive parallel sequencing ; African
%K BENIN
%M ISI:000452818000013
%P 108-127
%R 10.1016/j.molimm.2018.08.016
%U https://www.documentation.ird.fr/hor/fdi:010074796
%> https://www.documentation.ird.fr/intranet/publi/2018/12/010074796.pdf
%V 104
%W Horizon (IRD)
%X HLA-G/E/F genes exhibit immunomodulatory properties and are expressed in placenta. Little attention has been devoted to the study of these genes in sub-Saharan African populations, which are yet the most diverse. To fill this gap, we evaluated the complete gene variability, approximately 5.1 kb for HLA-G (n = 149), 7.7 kb for HLAE (n = 150) and 6.2 kb for HLA-P (n = 152) in the remote Beninese Toffin population, using massive parallel sequencing. Overall, 96, 37 and 68 variable sites were detected along the entire HLA-G,-E and-F, respectively, arranged into region-specific haplotypes; i.e., promoter haplotypes (16, 19, and 15 respectively), coding haplotypes (19, 15, and 29 respectively), 3' untranslated region (3'UTR) haplotypes (12, 7 and 2, respectively) and extended haplotypes (33, 31 and 32 respectively). All promoter/coding/3'UTR haplotypes followed the patterns already described in worldwide populations. HLA-E was the most conserved, exhibiting mainly two full-length encoded-molecules (E*01:01 and E*01:03), followed by HLA-F, three full-length proteins (F*01:01, F*01:02 and F*01:03) and HLA-G, four proteins: three full-length (G*01:01, G*01:03 and G*01:04) and one truncated (G*01:05N). Although HLA-G/E/F alleles in the Toffin population were the most frequently observed worldwide, the frequencies of the coding haplotypes were closely similar to those described for other African populations (Guinea-Conakry and Burkina-Faso), when compared to non-African ones (Brazilian), indicating that variable sites along these genes were present in Africa before human dispersion.
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