@article{fdi:010074609,
  title = {{SNP} discovery and molecular evolution in {A}nopheles gambiae, with special emphasis on innate immune system},
  author = {{C}ohuet, {A}nna and {K}rishnakumar, {S}. and {S}imard, {F}rederic and {M}orlais, {I}sabelle and {K}outsos, {A}. and {F}ontenille, {D}idier and {M}indrinos, {M}. and {K}afatos, {F}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {A}nopheles innate immunity affects {P}lasmodium development and is a potential target of innovative malaria control strategies. {T}he extent and distribution of nucleotide diversity in immunity genes might provide insights into the evolutionary forces that condition pathogen-vector interactions. {T}he discovery of polymorphisms is an essential step towards association studies of susceptibility to infection. {R}esults: {W}e sequenced coding fragments of 72 immune related genes in natural populations of {A}nopheles gambiae and of 37 randomly chosen genes to provide a background measure of genetic diversity across the genome. {M}ean nucleotide diversity (π) was 0.0092 in the {A}. gambiae {S} form, 0.0076 in the {M} form and 0.0064 in {A}. arabiensis. {W}ithin each species, no statistically significant differences in mean nucleotide diversity were detected between immune related and non immune related genes. {S}trong purifying selection was detected in genes of both categories, presumably reflecting strong functional constraints. {C}onclusion: {O}ur results suggest similar patterns and rates of molecular evolution in immune and non-immune genes in {A}. gambiae. {T}he 3,214 {S}ingle {N}ucleotide {P}olymorphisms ({SNP}s) that we identified are the first large set of {A}nopheles {SNP}s from fresh, field-collected material and are relevant markers for future phenotype-association studies.},
  keywords = {{CAMEROUN} ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {9},
  numero = {1},
  pages = {227},
  ISSN = {1471-2164},
  year = {2008},
  DOI = {10.1186/1471-2164-9-227},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074609},
}