Malele I. auteur aut IRD Nyingilili H. auteur aut IRD Lyaruu E. auteur aut IRD Tauzin M. auteur aut IRD Ollivier Bernard auteur aut IRD Cayol J. L. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Geiger Anne auteur aut IRD text journalArticle eng text/pdf reformatted digital access Background: Glossina pallidipes is a haematophagous insect that serves as a cyclic transmitter of trypanosomes causing African Trypanosomiasis (AT). To fully assess the role of G. pallidipes in the epidemiology of AT, especially the human form of the disease (HAT), it is essential to know the microbial diversity inhabiting the gut of natural fly populations. This study aimed to examine the diversity of G. pallidipes fly gut bacteria by culture-dependent approaches. Results: 113 bacterial isolates were obtained from aerobic and anaerobic microorganisms originating from the gut of G. pallidipes. 16S rDNA of each isolate was PCR amplified and sequenced. The overall majority of identified bacteria belonged in descending order to the Firmicutes (86.6%), Actinobacteria (7.6%), Proteobacteria (5.5%)and Bacteroidetes (0.3%). Diversity of Firmicutes was found higher when enrichments and isolation were performed under anaerobic conditions than aerobic ones. Experiments conducted in the absence of oxygen (anaerobiosis) led to the isolation of bacteria pertaining to four phyla (83% Firmicutes, 15% Actinobacteria, 1% Proteobacteria and 0.5% Bacteroidetes, whereas those conducted in the presence of oxygen (aerobiosis) led to the isolation of bacteria affiliated to two phyla only (90% Firmicutes and 10% Proteobacteria). Phylogenetic analyses placed these isolates into 11 genera namely Bacillus, Acinetobacter, Mesorhizobium, Paracoccus, Microbacterium, Micrococcus, Arthrobacter, Corynobacterium, Curtobacterium, Vagococcus and Dietzia spp.which are known to be either facultative anaerobes, aerobes, or even microaerobes. Conclusion: This study shows that G. pallidipes fly gut is an environmental reservoir for a vast number of bacterial species, which are likely to be important for ecological microbial well being of the fly and possibly on differing vectorial competence and refractoriness against AT epidemiology. specialized Facultative anaerobes Aerobes Microaerobes Bacterial diversity Gut Sleeping sickness Glossina pallidipes Tanzania TANZANIE 052 084 Abd-Alla A.M.M ed. edt IRD Tsiamis G. ed. edt IRD Boucias D.G. ed. edt IRD 18 Suppl. 1 art. 164 [10 ] 2018 1471-2180 https://www.documentation.ird.fr/hor/fdi:010074483 10.1186/s12866-018-1288-3 1471-2180 [F B010074483] https://www.documentation.ird.fr/hor/fdi:010074483 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-12/010074483.pdf IRD - Base Horizon / Pleins textes 2019-01-10 2023-07-11 fdi:010074483 fre