@article{fdi:010074483,
  title = {{B}acterial diversity obtained by culturable approaches in the gut of {G}lossina pallidipes population from a non sleeping sickness focus in {T}anzania : preliminary results},
  author = {{M}alele, {I}. and {N}yingilili, {H}. and {L}yaruu, {E}. and {T}auzin, {M}. and {O}llivier, {B}ernard and {C}ayol, {J}. {L}. and {F}ardeau, {M}arie-{L}aure and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {G}lossina pallidipes is a haematophagous insect that serves as a cyclic transmitter of trypanosomes causing {A}frican {T}rypanosomiasis ({AT}). {T}o fully assess the role of {G}. pallidipes in the epidemiology of {AT}, especially the human form of the disease ({HAT}), it is essential to know the microbial diversity inhabiting the gut of natural fly populations. {T}his study aimed to examine the diversity of {G}. pallidipes fly gut bacteria by culture-dependent approaches. {R}esults: 113 bacterial isolates were obtained from aerobic and anaerobic microorganisms originating from the gut of {G}. pallidipes. 16{S} r{DNA} of each isolate was {PCR} amplified and sequenced. {T}he overall majority of identified bacteria belonged in descending order to the {F}irmicutes (86.6%), {A}ctinobacteria (7.6%), {P}roteobacteria (5.5%)and {B}acteroidetes (0.3%). {D}iversity of {F}irmicutes was found higher when enrichments and isolation were performed under anaerobic conditions than aerobic ones. {E}xperiments conducted in the absence of oxygen (anaerobiosis) led to the isolation of bacteria pertaining to four phyla (83% {F}irmicutes, 15% {A}ctinobacteria, 1% {P}roteobacteria and 0.5% {B}acteroidetes, whereas those conducted in the presence of oxygen (aerobiosis) led to the isolation of bacteria affiliated to two phyla only (90% {F}irmicutes and 10% {P}roteobacteria). {P}hylogenetic analyses placed these isolates into 11 genera namely {B}acillus, {A}cinetobacter, {M}esorhizobium, {P}aracoccus, {M}icrobacterium, {M}icrococcus, {A}rthrobacter, {C}orynobacterium, {C}urtobacterium, {V}agococcus and {D}ietzia spp.which are known to be either facultative anaerobes, aerobes, or even microaerobes. {C}onclusion: {T}his study shows that {G}. pallidipes fly gut is an environmental reservoir for a vast number of bacterial species, which are likely to be important for ecological microbial well being of the fly and possibly on differing vectorial competence and refractoriness against {AT} epidemiology.},
  keywords = {{F}acultative anaerobes ; {A}erobes ; {M}icroaerobes ; {B}acterial diversity ; {G}ut ; {S}leeping sickness ; {G}lossina pallidipes ; {T}anzania ; {TANZANIE}},
  booktitle = {{S}pecial issue on enhancing vector refractoriness to trypanosome infection-foreword},
  journal = {{BMC} {M}icrobiology},
  volume = {18},
  numero = {{S}uppl. 1},
  pages = {art. 164 [10 ]},
  ISSN = {1471-2180},
  year = {2018},
  DOI = {10.1186/s12866-018-1288-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074483},
}