@article{fdi:010074481,
  title = {{P}revalence of symbionts and trypanosome infections in tsetse flies of two villages of the "{F}aro and {D}eo" division of the {A}damawa region of {C}ameroon},
  author = {{K}ame-{N}gasse, {G}. {I}. and {N}jiokou, {F}. and {M}elachio-{T}anekou, {T}. {T}. and {F}arikou, {O}. and {S}imo, {G}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}setse flies are vectors of human and animal {A}frican trypanosomiasis. {I}n spite of many decades of chemotherapy and vector control, the disease has not been eradicated. {O}ther methods like the transformation of tsetse fly symbionts to render the fly refractory to trypanosome infection are being evaluated. {T}he aim of the present study was to evaluate the association between trypanosome infections and the presence of symbionts in these tsetse species. {T}setse flies were trapped in two villages of the "{F}aro and {D}eo" {D}ivision of the {A}damawa region of {C}ameroon. {I}n the field, tsetse fly species were identified and their infection by trypanosomes was checked by microscopy. {I}n the laboratory, {DNA} was extracted from their midguts and the presence of symbionts ({S}odalis glossinidius and {W}olbachia sp.) and trypanosomes was checked by {PCR}. {S}ymbionts/trypanosomes association tests were performed. {R}esults: {T}hree tsetse fly species including {G}lossina tachinoides (90.1%), {G}lossina morsitans submorsitans (9.4%) and {G}lossina fuscipes fuscipes (0.5%) were caught. {I}n all the population we obtained an occurrence rate of 37.2% for {S}odalis glossinidius and 67.6% for {W}olbachia irrespective to tsetse flies species. {S}. glossinidius and {W}olbachia sp. occurrence rates were respectively 37 and 68% for {G}. tachinoides and 28.6 and 59.5% for {G}. m. submorsitans. {B}etween {G}olde {B}ourle and {M}ayo {D}agoum significant differences were observed in the prevalence of symbionts. {P}revalence of trypanosomes were 34.8% for {G}lossina tachinoides and 40.5% for {G}lossina morsitans submorsitans. {I}n {G}. tachinoides, the trypanosome infection rates were 11, 2.6 and 13.7%, respectively, for {T}. brucei s.l., {T}. congolense forest type and {T}. congolense savannah type. {I}n {G}. m. submorsitans, these infection rates were 16.7, 9.5 and, 2.4% respectively, for {T}. brucei s.l., {T}. congolense forest type and {T}. congolense savannah type. {C}onclusions: {T}he rate of tsetse fly infection by trypanosomes was low compared to those obtained in {HAT} foci of south {C}ameroon, and this rate was not statistically linked to the rate of symbiont occurrence. {T}his study allowed to show for the first time the presence of {W}olbachia sp. in the tsetse fly sub-species {G}lossina morsitans submorsitans and {G}lossina tachinoides.},
  keywords = {{A}nimal {A}frican trypanosomiasis ; {T}rypanosoma spp. ; {S}ymbionts ; {T}setse flies ; {T}ripartite interactions ; "{F}aro and {D}eo" division ; {C}ameroon ; {CAMEROUN}},
  booktitle = {{S}pecial issue on enhancing vector refractoriness to trypanosome infection-foreword},
  journal = {{BMC} {M}icrobiology},
  volume = {18},
  numero = {{S}uppl. 1},
  pages = {art. 159 [9 p.]},
  ISSN = {1471-2180},
  year = {2018},
  DOI = {10.1186/s12866-018-1286-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074481},
}