@article{fdi:010074397,
  title = {{M}utations in {G}lu{C}l associated with field ivermectin-resistant head lice from {S}enegal},
  author = {{A}manzougaghene, {N}. and {F}enollar, {F}. and {D}iatta, {G}eorges and {S}okhna, {C}heikh and {R}aoult, {D}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{T}hrough its unique mode of action, ivermectin represents a relatively new and very promising tool to fight against human lice, especially in cases of resistance to commonly used pediculicides. {H}owever, ivermectin resistance in the field has already begun to be reported. {T}herefore, understanding the mechanisms involved is a key step in delaying and tackling this phenomenon. {I}n this study, field head lice with confirmed clinical resistance to ivermectin in rural human populations from {S}enegal were subjected to genetic analysis targeting the {G}lu{C}l gene, the primary target of ivermectin known to be involved in resistance. {T}hrough {DNA}-polymorphism analysis, three relevant non-synonymous mutations in {G}lu{C}l which were found only in ivermectin-resistant head lice (76 head lice tested), were identified. {T}he {A}251{V} mutation found in the {TM}3 transmembrane domain was the most prevalent (allelic frequency of 0.33), followed by the {S}46{P} mutation (0.28) located at the {N}-terminal extracellular domain. {T}he {H}272{R} was in the {M}3-{M}4 linker transmembrane region of {G}lu{C}l and has shown the lowest frequency (0.18). {P}olymerase chain reaction restriction fragment length polymorphism ({PCRRFLP}) diagnostic assays were also developed for their accurate genotyping. {O}ur study is the first to report the presence of {G}lu{C}l point mutations in clinical ivermectin-resistant head lice occurring in rural human populations of {S}enegal.},
  keywords = {{H}ead lice ; {G}lu{C}l ; {I}vermectin ; {S}enegal ; {R}esistance ; {SENEGAL}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {A}ntimicrobial {A}gents},
  volume = {52},
  numero = {5},
  pages = {593--598},
  ISSN = {0924-8579},
  year = {2018},
  DOI = {10.1016/j.ijantimicag.2018.07.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074397},
}