@article{fdi:010073703,
  title = {{F}unctional analysis of {A}frican {X}anthomonas oryzae pv. oryzae {TAL}omes reveals a new susceptibility gene in bacterial leaf blight of rice},
  author = {{T}ran, {T}. {T}. and {P}erez-{Q}uintero, {A}. {L}. and {W}onni, {I}. and {C}arpenter, {S}. {C}. {D}. and {Y}u, {Y}. {H}. and {W}ang, {L}. and {L}each, {J}. {E}. and {V}erdier, {V}al{\'e}rie and {C}unnac, {S}{\'e}bastien and {B}ogdanove, {A}. {J}. and {K}oebnik, {R}alf and {H}utin, {M}. and {S}zurek, {B}oris},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ost {X}anthomonas species translocate {T}ranscription {A}ctivator-{L}ike ({TAL}) effectors into plant cells where they function like plant transcription factors via a programmable {DNA}-binding domain. {C}haracterized strains of rice pathogenic {X}. oryzae pv. oryzae harbor 9-16 different tal effector genes, but the function of only a few of them has been decoded. {U}sing sequencing of entire genomes, we first performed comparative analyses of the complete repertoires of {TAL} effectors, herein referred to as {TAL}omes, in three {X}oo strains forming an {A}frican genetic lineage different from {A}sian {X}oo. {A} phylogenetic analysis of the three {TAL}omes combined with in silico predictions of {TAL} effector targets showed that {A}frican {X}oo {TAL}omes are highly conserved, genetically distant from {A}sian ones, and closely related to {TAL} effectors from the bacterial leaf streak pathogen {X}anthomonas oryzae pv. oryzicola ({X}oc). {N}ine clusters of {TAL} effectors could be identified among the three {TAL}omes, including three showing higher levels of variation in their repeat variable diresidues ({RVD}s). {D}etailed analyses of these groups revealed recombination events as a possible source of variation among {TAL} effector genes. {N}ext, to address contribution to virulence, nine {TAL} effector genes from the {M}alian {X}oo strain {MAI}1 and four allelic variants from the {B}urkinabe {X}oo strain {BAI}3, thus representing most of the {TAL} effector diversity in {A}frican {X}oo strains, were expressed in the {TAL} effector-deficient {X}. oryzae strain {X}11-5{A} for gain-of-function assays. {I}noculation of the susceptible rice variety {A}zucena lead to the discovery of three {TAL} effectors promoting virulence, including two {TAL} effectors previously reported to target the susceptibility ({S}) gene {O}s{SWEET}14 and a novel major virulence contributor, {T}al{B}. {RNA} profiling experiments in rice and in silico prediction of {EBE}s were carried out to identify candidate targets of {T}al{B}, revealing {O}s{TFX}1, a b{ZIP} transcription factor previously identified as a bacterial blight {S} gene, and {O}s{ERF}#123, which encodes a subgroup {IX}c {AP}2/{ERF} transcription factor. {U}se of designer {TAL} effectors demonstrated that induction of either gene resulted in greater susceptibility to strain {X}11-5{A}. {T}he induction of {O}s{ERF}$123 by {BAI}3 {D}elta 1, a tal{B} knockout derivative of {BAI}3, carrying these designer {TAL} effectors increased virulence of {BAI}3 {D}elta 1, validating {O}s{ERF}#123 as a new, bacterial blight {S} gene.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {P}athogens},
  volume = {14},
  numero = {6},
  pages = {e1007092 [25 ]},
  ISSN = {1553-7366},
  year = {2018},
  DOI = {10.1371/journal.ppat.1007092},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073703},
}