Zghal R. Z. auteur aut IRD Kharrat M. auteur aut IRD Rebai A. auteur aut IRD Ben Khedher S. auteur aut IRD Jallouli W. auteur aut IRD Elleuch J. auteur aut IRD Ginibre Carole auteur aut IRD Chandre Fabrice auteur aut IRD Tounsi S. auteur aut IRD text journalArticle eng text/pdf born digital access Bacillus thuringiensis israelensis biological larvicides represent a good approach to support vector borne diseases control programs targeting earlier aquatic larval stages to limit their adult mosquito spreading. BUPM98, a Tunisian B. thuringiensis israelensis strain, is highly active against Aedes aegypti larvae. For the production of BUPM98 bioinsecticide, an agricultural by-product medium consisting of gruel and soybean meal has been optimized. Optimization of the medium composition was performed with statistical methodology based on experimental designs. Accordingly, the optimum toxin production was 2375.36 +/- 48.52 mg/L at the following conditions: K2HPO4: 60 mM, pH: 7.6 and soybean meal: 32 g/L, which led to an improvement of 30% compared to the starting conditions. The impact of water quality on the effectiveness of BUPM98 delta-endotoxins against Culex pipiens larvae was investigated. Indeed, under field conditions, the direct application of BUPM98 fermentation product in stagnant water pools reduced the immature density of C. pipiens larvae by more than 95% after 48 h of treatment. Prolonged residual action was observed until 14 days. In conclusion, BUPM98, a local strain of B. thuringiensis israelensis, is very promising bioinsecticide for mosquito population management in Tunisia. specialized Bacillus thuringiensis israelensis Medium optimization Aedes aegypti Culex pipens Mosquito's control in the field TUNISIE SFAX 052 084 020 124 46-52 2018 1049-9644 https://www.documentation.ird.fr/hor/fdi:010073184 10.1016/j.biocontrol.2018.06.002 1049-9644 [F B010073184] https://www.documentation.ird.fr/hor/fdi:010073184 https://www.documentation.ird.fr/intranet/publi/2018/07/010073184.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2018-09-03 2023-07-11 fdi:010073184 fre