@article{fdi:010073161, title = {{T}issue distribution and elimination of ciguatoxins in {T}ridacna maxima ({T}ridacnidae, {B}ivalvia) fed {G}ambierdiscus polynesiensis}, author = {{R}ou{\'e}, {M}{\'e}lanie and {D}arius, {H}. {T}. and {U}ng, {A}. and {V}iallon, {J}. and {S}ibat, {M}. and {H}ess, {P}. and {A}mzil, {Z}. and {C}hinain, {M}.}, editor = {}, language = {{ENG}}, abstract = {{C}iguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins ({CTX}s). {C}iguatera-like poisoning events involving giant clams ({T}ridacna maxima) are reported occasionally from {P}acific islands communities. {T}he present study aimed at providing insights into {CTX}s tissue distribution and detoxification rate in giant clams exposed to toxic cells of {G}ambierdiscus polynesiensis, in the framework of seafood safety assessment. {I}n a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay ({CBA}-{N}2a) and liquid chromatography-tandem mass spectrometry ({LC}-{MS}/{MS}) analyses. {T}he viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. {A}ll tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. {I}n a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). {O}bservations suggest that no toxin elimination is visible in {T}. maxima throughout 6 days of detoxification.}, keywords = {giant clams ; ex situ exposure to toxic algae ; ciguatoxins ; {G}ambierdiscus polynesiensis ; anatomical distribution ; toxin elimination ; {CBA}-{N}2a ; {LC}-{MS}/{MS}}, booktitle = {}, journal = {{T}oxins}, volume = {10}, numero = {5}, pages = {art. 189 [13p.]}, ISSN = {2072-6651}, year = {2018}, DOI = {10.3390/toxins10050189}, URL = {https://www.documentation.ird.fr/hor/fdi:010073161}, }