@article{fdi:010073054,
  title = {{D}etection of {J}apanese {E}ncephalitis {V}irus {RNA} in human throat samples in {L}aos : a pilot study},
  author = {{B}harucha, {T}. and {S}engvilaipaseuth, {O}. and {S}eephonelee, {M}. and {V}ongsouvath, {M}. and {V}ongsouvath, {M}. and {R}attanavong, {S}. and {P}iorkowski, {G}. and {L}ecuit, {M}. and {G}orman, {C}. and {P}ommier, {J}. {D}. and {N}ewton, {P}. {N}. and de {L}amballerie, {X}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey},
  editor = {},
  language = {{ENG}},
  abstract = {{J}apanese encephalitis virus ({JEV}) is the most commonly identified cause of acute encephalitis syndrome ({AES}) in {A}sia. {T}he {WHO} recommended test is anti-{JEV} {I}g{M}-antibody-capture-enzyme-linked-immunosorbent- assay ({JEV} {MAC}-{ELISA}). {H}owever, data suggest this has low positive predictive value, with false positives related to other {F}lavivirus infections and vaccination. {JEV} {RT}-{PCR} in cerebrospinal fluid ({CSF}) and/or serum is highly specific, but is rarely positive; 0-25% of patients that fulfil the {WHO} definition of {JE} (clinical {A}cute {E}ncephalitis {S}yndrome ({AES}) and {JEV} {MAC}-{ELISA} positive). {T}esting other body fluids by {JEV} {RT}-q{PCR} may improve the diagnosis. {A}s a pilot study thirty patients admitted to {M}ahosot {H}ospital 2014-2017, recruited to the {S}outh-{E}ast-{A}sia-{E}ncephalitis study, were tested by {JEV} {MAC}-{ELISA} and two {JEV} real-time {RT}-{PCR} ({RT}-q{PCR}) assays ({NS}2{A} and {NS}3). {E}leven (36.7%) were {JEV} {MAC}-{ELISA} positive. {A}vailable {CSF} and serum samples of these patients were {JEV} {RT}-q{PCR} negative but 2 (7%) had {JEV} {RNA} detected in their throat swabs. {JEV} {RNA} was confirmed by re-testing, and sequencing of {RT}-q{PCR} products. {A}s the first apparent report of {JEV} {RNA} detection in human throat samples, the provides new perspectives on human {JEV} infection, potentially informing improving {JEV} detection. {W}e suggest that testing patients' throat swabs for {JEV} {RNA} is performed, in combination with molecular and serological {CSF} and serum investigations, on a larger scale to investigate the epidemiology of the presence of {JEV} in human throats. {T}hroat swabs are an easy and non-invasive tool that could be rolled out to a wider population to improve knowledge of {JEV} molecular epidemiology.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {8},
  numero = {},
  pages = {art. 8018 [5 p.]},
  ISSN = {2045-2322},
  year = {2018},
  DOI = {10.1038/s41598-018-26333-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073054},
}