@article{fdi:010072488,
  title = {{I}dentification of a hypervirulent pathotype of {R}ice yellow mottle virus : a threat to genetic resistance deployment in {W}est-{C}entral {A}frica},
  author = {{H}{\'e}brard, {E}ug{\'e}nie and {G}alzi, {A}gn{\`e}s and {O}ludare, {A}. and {P}oulicard, {N}ils and {A}ribi, {J}amel and {F}abre, {S}. and {I}ssaka, {S}. and {M}ariac, {C}{\'e}dric and {D}ereeper, {A}lexis and {A}lbar, {L}aurence and {S}ilue, {D}. and {F}argette, {D}enis},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice yellow mottle virus ({RYMV}) causes high losses to rice production in {A}frica. {S}everal sources of varietal high resistance are available but the emergence of virulent pathotypes that are able to overcome one or two resistance alleles can sometimes occur. {B}oth resistance spectra and viral adaptability have to be taken into account to develop sustainable rice breeding strategies against {RYMV}. {I}n this study, we extended previous resistance spectrum analyses by testing the rymv1-4 and rymv1-5 alleles that are carried by the rice accessions {T}og5438 and {T}og5674, respectively, against isolates that are representative of {RYMV} genetic and pathogenic diversity. {O}ur study revealed a hypervirulent pathotype, named thereafter pathotype {T}9, that is able to overcome all known sources of high resistance. {T}his pathotype, which is spatially localized in {W}est-{C}entral {A}frica, appears to be more abundant than previously suspected. {T}o better understand the adaptive processes of pathotype {T}9, molecular determinants of resistance breakdown were identified via {S}anger sequencing and validated through directed mutagenesis of an infectious clone. {T}hese analyses confirmed the key role of convergent nonsynonymous substitutions in the central part of the viral genome-linked protein to overcome {RYMV}1-mediated resistance. {I}n addition, deep-sequencing analyses revealed that resistance breakdown does not always coincide with fixed mutations. {A}ctually, virulence mutations that are present in a small proportion of the virus population can be sufficient for resistance breakdown. {C}onsidering the spatial distribution of {RYMV} strains in {A}frica and their ability to overcome the {RYMV} resistance genes and alleles, we established a resistance-breaking risk map to optimize strategies for the deployment of sustainable and resistant rice lines in {A}frica.},
  keywords = {{AFRIQUE}},
  booktitle = {},
  journal = {{P}hytopathology},
  volume = {108},
  numero = {2},
  pages = {299--307},
  ISSN = {0031-949{X}},
  year = {2018},
  DOI = {10.1094/phyto-05-17-0190-r},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072488},
}