Structure and distribution of centromeric retrotransposons at diploid and allotetraploid Coffea centromeric and pericentromeric regions de Castro Nunes R. auteur aut IRD Orozco-Arias S. auteur aut IRD Crouzillat D. auteur aut IRD Mueller L. A. auteur aut IRD Strickler S. R. auteur aut IRD Descombes P. auteur aut IRD Fournier C. auteur aut IRD Moine D. auteur aut IRD Kochko Alexandre de auteur aut IRD Yuyama P. M. auteur aut IRD Vanzela A. L. L. auteur aut IRD Guyot Romain auteur aut IRD text journalArticle eng text/pdf reformatted digital access Centromeric regions of plants are generally composed of large array of satellites from a specific lineage of Gypsy LTR-retrotransposons, called Centromeric Retrotransposons. Repeated sequences interact with a specific H3 histone, playing a crucial function on kinetochore formation. To study the structure and composition of centromeric regions in the genus Coffea, we annotated and classified Centromeric Retrotransposons sequences from the allotetraploid C. arabica genome and its two diploid ancestors: Coffea canephora and C. eugenioides. Ten distinct CRC (Centromeric Retrotransposons in Coffea) families were found. The sequence mapping and FISH experiments of CRC Reverse Transcriptase domains in C. canephora, C. eugenioides, and C. arabica clearly indicate a strong and specific targeting mainly onto proximal chromosome regions, which can be associated also with heterochromatin. PacBio genome sequence analyses of putative centromeric regions on C. arabica and C. canephora chromosomes showed an exceptional density of one family of CRC elements, and the complete absence of satellite arrays, contrasting with usual structure of plant centromeres. Altogether, our data suggest a specific centromere organization in Coffea, contrasting with other plant genomes. specialized coffee CRM lineages FISH Gypsy pseudochromosomes proximal chromosome regions centromeres 076 Frontiers in Plant Science 9 art. 175 [14p.] 2018 1664-462X https://www.documentation.ird.fr/hor/fdi:010072372 10.3389/fpls.2018.00175 1664-462X [F B010072372] https://www.documentation.ird.fr/hor/fdi:010072372 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-03/010072372.pdf IRD - Base Horizon / Pleins textes 2018-04-03 2023-07-11 fdi:010072372 fre